Chemiluminescent flow-through sensor for hydrogen peroxide based on sol-gel immobilized hemoglobin as catalyst

被引：33

作者：

Li, BX ^{[1
]}

Zhang, ZJ ^{[1
]}

Zhao, LX ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Dept Chem, Xian 710062, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 445卷 / 02期

基金：

中国国家自然科学基金;

关键词：

chemiluminescence; flow-through sensor; sol-gel method; hydrogen peroxide; hemoglobin;

D O I：

10.1016/S0003-2670(01)01284-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel chemiluminescence (CL) flow-through sensor for hydrogen peroxide is described. It is prepared by immobilizing hemoglobin (Hb) with the sol-gel method on the flow cell, and by electrostatically immobilizing luminol on strongly basic anion-exchange resin. Hydrogen peroxide is sensed by the CL reaction with Hb sensitive membrane and luminol bleeding from the ion-exchange column with immobilized luminol by hydrolysis. The calibration graph is linear in the range of 6 x 10(-5) to 4 x 10(-7) mol l(-1) with a detection limit of 1.3 x 10(-7) mol l(-1) (3 sigma). A complete analysis could be performed in I min with a relative standard deviation of 1.7% for 5 x 10(-6) mol l(-1) H2O2 (n = 9). The sensor has been applied successfully to the determination of hydrogen peroxide in rainwater. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：161 / 167

页数：7

共 30 条

[1] CI Y, 1990, TALANTA, V37, P63
[2] Dithiobissuccinimidyl propionate as an anchor for assembling peroxidases at electrodes surfaces and its application in a H2O2 biosensor
Darder, M
Takada, K
Pariente, F
Lorenzo, E
Abruña, HD
[J]. ANALYTICAL CHEMISTRY, 1999, 71 (24) : 5530 - 5537
[3] Díaz AN, 1998, ANAL CHIM ACTA, V363, P221
[4] FLOW-THROUGH OPTICAL BIOSENSOR BASED ON THE PERMANENT IMMOBILIZATION OF AN ENZYME AND TRANSIENT RETENTION OF A REACTION-PRODUCT
FERNANDEZROMERO, JM
DECASTRO, MDL
[J]. ANALYTICAL CHEMISTRY, 1993, 65 (21) : 3048 - 3052
[5] Hydrogen peroxide sensitive biosensor based on plant peroxidases entrapped in Os-modified polypyrrole films
Gaspar, S
Habermüller, K
Csöregi, E
Schuhmann, W
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2001, 72 (01) : 63 - 68
[6] GENFA Z, 1992, ANAL CHEM, V64, P517
[7] Chemiluminescent flow sensor for H2O2 based on the decomposition of H2O2 catalyzed by cobalt(II)-ethanolamine complex immobilized on resin
Hanaoka, S
Lin, JM
Yamada, M
[J]. ANALYTICA CHIMICA ACTA, 2001, 426 (01) : 57 - 64
[8] Catalytic determination of hydrogen peroxide by using the molybdenum-porphyrin complex as a mimetic enzyme of peroxidase
Huang, XM
Zhu, M
Mao, LY
Shen, HX
[J]. ANALYTICAL SCIENCES, 1997, 13 (01) : 145 - 147
[9] Chemiluminescence flow biosensor for hydrogen peroxide with immobilized reagents
Li, BX
Zhang, ZJ
Jin, Y
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2001, 72 (02) : 115 - 119
[10] Silica sol-gel immobilized amperometric biosensor for hydrogen peroxide
Li, J
Tan, SN
Ge, HL
[J]. ANALYTICA CHIMICA ACTA, 1996, 335 (1-2) : 137 - 145

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