Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli

被引：111

作者：

Kim, P ^{[1
]}

Laivenieks, M ^{[1
]}

Vieille, C ^{[1
]}

Zeikus, JG ^{[1
]}

机构：

[1] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 02期

关键词：

D O I：

10.1128/AEM.70.2.1238-1241.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.

引用

页码：1238 / 1241

页数：4

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