Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction

被引：61

作者：

Song, Suquan ^{[1
]}

Wu, Aibo ^{[1
]}

Shi, Xizhi ^{[1
]}

Li, Rongxiu ^{[1
]}

Lin, Zhixin ^{[1
]}

Zhang, Dabing ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Minist Educ, Coll Life Sci & Technol, Bor Luh Food Safety Ctr,Key Lab Microbial Metab, Shanghai 200240, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 390卷 / 08期

关键词：

molecularly imprinted polymers; erythromycin; solid-phase extraction; pig muscle; tissue sample;

D O I：

10.1007/s00216-008-1985-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Six molecularly imprinted polymers (MIPs) of erythromycin (ERY) were prepared by noncovalent bulk polymerization using methacrylic acid (MAA) as the functional monomer. On the basis of binding analysis, the MIPs with 1:2 optimum ratio of template to MAA were selected for subsequent scanning electron microscopy and Brunauer-Emmett-Teller analyses, which indicated that the MIPs had more convergent porous structures than the nonimprinted polymers. The equilibrium binding experiments showed that the binding sites of MIPs were heterogeneous, with two dissociation constants of 0.005 and 0.63 mg mL(-1), respectively. Furthermore, the performance of the MIPs as solid-phase extraction (SPE) sorbents was evaluated, and the selectivity analysis showed that the MIPs could recognize ERY with moderate cross-reactivity for other macrolides. The overall investigation of molecularly imprinted SPE for cleanup and enrichment of the ERY in pig muscle and tap water confirmed the feasibility of utilizing the MIPs obtained as specific SPE sorbents for ERY extraction in real samples.

引用

页码：2141 / 2150

页数：10

共 37 条

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