Visual cycle retinoid processing proteins are present in HEK293S cells

被引：15

作者：

Chen, YM ^{[1
]}

Moiseyev, G ^{[1
]}

Wu, BX ^{[1
]}

Ma, JX ^{[1
]}

Crouch, RK ^{[1
]}

机构：

[1] Med Univ S Carolina, Dept Ophthalmol, Charleston, SC 29425 USA

来源：

VISION RESEARCH | 2003年 / 43卷 / 28期

基金：

美国国家卫生研究院;

关键词：

LRAT; HEK293; retinol esterification; visual cycle;

D O I：

10.1016/j.visres.2003.08.001

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In HEK293S cells expressing opsin, rhodopsin regenerates on addition of all-trans retinol. This study was to determine if key proteins in the retinal pigment epithelium (RPE) are present in these cells. Cellular retinoid binding protein, cellular retinoic-acid binding protein, RPE65, caveolin-1-alpha- and -beta-isoforms, interphotoreceptor retinoid binding protein, and 11-cis retinol dehydrogenase. but not lecithin: retinol acyltransferase (LRAT), were identified by Western blot analysis. LRAT transcripts were found by RT-PCR and Southern blot analysis. Small interference RNA specific to LRAT reduced ester formation, confirming that the enzyme is present. Therefore, HEK293S cells contain the functional components of the retinoid cycle found in the RPE. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：3037 / 3044

页数：8

共 28 条

[1] SEPARATION BY PROGRAMMED-GRADIENT HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF VITAMIN A ISOMERS, THEIR ESTERS, ALDEHYDES, OXIMES AND VITAMIN-A2 - PRESENCE OF RETINYL ESTER IN DARK-ADAPTED GOLDFISH PIGMENT-EPITHELIUM
BRIDGES, CDB
FONG, SL
ALVAREZ, RA
[J]. VISION RESEARCH, 1980, 20 (04) : 355 - 360
[2] HEK293S cells have functional retinoid processing machinery
Brueggemann, LI
Sullivan, JM
[J]. JOURNAL OF GENERAL PHYSIOLOGY, 2002, 119 (06) : 593 - 612
[3] all-trans-retinal forms a visible-absorbing pigment with human rod opsin
Brueggemann, LI
Sullivan, JM
[J]. BIOCHEMISTRY, 2001, 40 (14) : 4446 - 4453
[4] SUBSTRATE SPECIFICITIES AND MECHANISM IN THE ENZYMATIC PROCESSING OF VITAMIN-A INTO 11-CIS-RETINOL
CANADA, FJ
LAW, WC
RANDO, RR
YAMAMOTO, T
DERGUINI, F
NAKANISHI, K
[J]. BIOCHEMISTRY, 1990, 29 (41) : 9690 - 9697
[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
CHOMCZYNSKI, P
SACCHI, N
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
[6] Crabb JW, 1998, METH MOL B, V89, P91
[7] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, SM
Harborth, J
Lendeckel, W
Yalcin, A
Weber, K
Tuschl, T
[J]. NATURE, 2001, 411 (6836) : 494 - 498
[8] All-trans-retinyl esters are the substrates for isomerization in the vertebrate visual cycle
Gollapalli, DR
Rando, RR
[J]. BIOCHEMISTRY, 2003, 42 (19) : 5809 - 5818
[9] Guo XJ, 2001, CANCER RES, V61, P2774
[10] HAMEL CP, 1993, J BIOL CHEM, V268, P15751

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