Somatic embryogenesis and long term maintenance of embryogenic lines from fox grapes

被引：37

作者：

Motoike, SY ^{[1
]}

Skirvin, RM ^{[1
]}

Norton, MA ^{[1
]}

Otterbacher, AG ^{[1
]}

机构：

[1] Univ Illinois, Dept Nat Resources & Environm Sci, EDMML 258, Urbana, IL 61801 USA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2001年 / 66卷 / 02期

关键词：

charcoal; embryo conversion; embryo germination; embryo regeneration; Fredonia; grapes; Niagara; polyethylene glycol; VitisxLabruscana LH Bailey;

D O I：

10.1023/A:1010604628865

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present paper, a method for the induction and long-term maintenance embryogenic cultures for Vitis x Labruscana 'Niagara' and 'Fredonia' is reported. Embryogenic cultures from these two cultivars were induced in an embryogenesis establishment medium (CIM) from ovaries obtained from flowers 10-14 days pre-anthesis. The embryogenic lines obtained in this experiment have been stably maintained for more than 2 years, through repeated subcultures on a long-term maintenance medium (LTMM) without loss of embryogenic competence. Somatic embryo regeneration and maturation have been successfully achieved after 30 days of cultivating embryogenic cultures in an embryo regeneration medium (EDMM), supplemented with charcoal and polyethylene glycol. The somatic embryos were successfully germinated in two different media, 'Fredonia' germination medium (FGM) and 'Niagara' germination medium (NGM), and converted into normal looking plants on a conversion medium (CM).

引用

页码：121 / 131

页数：11

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