Somatic embryogenesis and long term maintenance of embryogenic lines from fox grapes

被引：37
作者：
Motoike, SY [1 ]
Skirvin, RM [1 ]
Norton, MA [1 ]
Otterbacher, AG [1 ]
机构：
[1] Univ Illinois, Dept Nat Resources & Environm Sci, EDMML 258, Urbana, IL 61801 USA
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2001年 / 66卷 / 02期
关键词：
charcoal; embryo conversion; embryo germination; embryo regeneration; Fredonia; grapes; Niagara; polyethylene glycol; VitisxLabruscana LH Bailey;
D O I：
10.1023/A:1010604628865
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In the present paper, a method for the induction and long-term maintenance embryogenic cultures for Vitis x Labruscana 'Niagara' and 'Fredonia' is reported. Embryogenic cultures from these two cultivars were induced in an embryogenesis establishment medium (CIM) from ovaries obtained from flowers 10-14 days pre-anthesis. The embryogenic lines obtained in this experiment have been stably maintained for more than 2 years, through repeated subcultures on a long-term maintenance medium (LTMM) without loss of embryogenic competence. Somatic embryo regeneration and maturation have been successfully achieved after 30 days of cultivating embryogenic cultures in an embryo regeneration medium (EDMM), supplemented with charcoal and polyethylene glycol. The somatic embryos were successfully germinated in two different media, 'Fredonia' germination medium (FGM) and 'Niagara' germination medium (NGM), and converted into normal looking plants on a conversion medium (CM).
引用
收藏
页码：121 / 131
页数：11
相关论文
共 29 条
  • [1] Plant transformation: Problems and strategies for practical application
    Birch, RG
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1997, 48 : 297 - 326
  • [2] CHEE R, 1983, VITIS, V22, P363
  • [3] SOMATIC EMBRYOGENESIS FROM GRAPEVINE CELLS - I-IMPROVEMENT OF EMBRYO DEVELOPMENT BY CHANGES IN CULTURE CONDITIONS
    COUTOSTHEVENOT, P
    GOEBELTOURAND, I
    MAURO, MC
    JOUANNEAU, JP
    BOULAY, M
    DELOIRE, A
    GUERN, J
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1992, 29 (02) : 125 - 133
  • [4] EFFECTS OF DESICCATION, MEDIUM OSMOLARITY AND ABSCISIC-ACID ON THE MATURATION OF HEVEA-BRASILIENSIS SOMATIC EMBRYOS
    ETIENNE, H
    MONTORO, P
    MICHAUXFERRIERE, N
    CARRON, MP
    [J]. JOURNAL OF EXPERIMENTAL BOTANY, 1993, 44 (267) : 1613 - 1619
  • [5] Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (Vitis vinifera L): Embryonic organogenesis
    Faure, O
    Aarrouf, J
    Nougarede, A
    [J]. ANNALS OF BOTANY, 1996, 78 (01) : 29 - 37
  • [6] Gray Dennis J., 1992, V8, P229
  • [7] GRAY DJ, 1995, BIOTECHNOLOGY AGR FO, V30, P126
  • [8] KIKKERT JR, 1997, ACTA HORTIC, V447, P273
  • [9] KOMAMINE A, 1992, IN VITRO CELL DEV-PL, V28P, P11
  • [10] LITZ RE, 1992, BIOTECHNOLOGY PERENN, P229
123