Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals

被引：129
|
作者：
Min, Jung-Joon [1 ,2 ]
Nguyen, Vu H. [1 ]
Kim, Hyun-Ju [3 ]
Hong, Yeongjin [3 ]
Choy, Hyon E. [3 ]
机构：
[1] Chonnam Natl Univ, Sch Med, Dept Nucl Med, Lab In Vivo Mol Imaging, Kwangju 501746, South Korea
[2] Gwangju Inst Sci & Technol, Bioimaging Res Ctr, Kwangju 500712, South Korea
[3] Chonnam Natl Univ, Sch Med, Dept Microbiol, Kwangju 501746, South Korea
来源：
NATURE PROTOCOLS | 2008年 / 3卷 / 04期
关键词：
D O I：
10.1038/nprot.2008.32
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a protocol for imaging bacterial luciferase (Lux)-expressing bacteria in small living animals. In this protocol, light emitted by Lux-expressing bacteria is detected and monitored by a cooled charge-coupled device detector. When these bacteria are administered to animals, it provides a potentially valuable approach to generate sensitive whole-body images with extremely low background. This imaging technology should enable the real-time monitoring of bacterial migration into both primary and metastatic tumors in several different mouse tumor models at a strong quantification power.
引用
收藏
页码：629 / 636
页数：8
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