Intracellular delivery of core-shell fluorescent silica nanoparticles

被引：154

作者：

Fuller, Jason E. ^{[1
]}

Zugates, Gregory T. ^{[1
]}

Ferreira, Lino S. ^{[1
,2
,3
]}

Ow, Hooisweng S. ^{[4
]}

Nguyen, Nicholas N. ^{[1
]}

Wiesner, Ulrich B. ^{[5
]}

Langer, Robert S. ^{[1
]}

机构：

[1] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[2] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal

[3] Biocant Biotechnol Innovat Ctr, P-3060197 Cantanhede, Portugal

[4] Hybrid Silica Technologies Inc, Ithaca, NY USA

[5] Cornell Univ, Dept Mat Sci & Engn, Ithaca, NY 14853 USA

来源：

BIOMATERIALS | 2008年 / 29卷 / 10期

关键词：

DNA; silica; surface modification; confocal microscopy; cell viability; drug delivery;

D O I：

10.1016/j.biomaterials.2007.11.025

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Highly fluorescent core-shell silica nanoparticles made by the modified Stober process (C dots) are promising as tools for sensing and imaging subcellular agents and structures but will only be useful if they can be easily delivered to the cytoplasm of the subject cells. This work shows that C dots can be electrostatically coated with cationic polymers, changing their surface charge and enabling them to escape from endosomes and enter the cytoplasm and nucleus. As an example of cellular delivery, we demonstrate that these particles can also be complexed with DNA and mediate and trace DNA delivery and gene expression. (c) 2007 Published by Elsevier Ltd.

引用

页码：1526 / 1532

页数：7

共 30 条

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