Ionic liquid-based aqueous two-phase system, a sample pretreatment procedure prior to high-performance liquid chromatography of opium alkaloids

被引：207

作者：

Li, SH ^{[1
]}

He, CY ^{[1
]}

Liu, HW ^{[1
]}

Li, K ^{[1
]}

Liu, F ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 826卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

aqueous two-phase systems; ionic liquid; extraction; opium alkaloids;

D O I：

10.1016/j.jchromb.2005.08.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An ionic liquid, 1-butyl-3-methylimidazolium chloride ([C(4)mim]Cl)/salt aqueous two-phase systems (ATPS) was presented as a simple, rapid and effective sample pretreatment technique coupled with high-performance liquid chromatography (HPLC) for analysis of the major opium alkaloids in Pericarpium papaveris. To find optimal conditions, the partition behaviors of codeine and papaverine in ionic liquid/salt aqueous two-phase systems were investigated. Various factors were considered systematically, and the results indicated that both the pH value and the salting-out ability of salt had great influence on phase separation. The recoveries of codeine and papaverine were 90.0-100.2% and 99.3-102.0%, respectively, from aqueous samples of P. papaveris by the proposed method. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：58 / 62

页数：5

共 22 条

[1] Aqueous two-phase partitioning sample preparation prior to liquid chromatography of hydrophilic drugs in blood [J].