Interaction of picornavirus 2C polypeptide with the viral negative-strand RNA

被引:29
作者
Banerjee, R [1 ]
Dasgupta, A [1 ]
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Microbiol Mol Genet & Immunol, Los Angeles, CA 90095 USA
关键词
D O I
10.1099/0022-1317-82-11-2621
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The picornavirus membrane-associated polypeptide 2C is believed to be required for viral RNA synthesis. Hepatitis A virus (HAV)- and human rhinovirus (HRV)-encoded recombinant 2C proteins have been expressed, purified and examined for their ability to interact with the terminal sequences of viral positive- and negative-strand RNAs. The results demonstrate that both the HAV- and the HRV-encoded 2C polypeptide specifically interact with the 3'-terminal sequences of the negative-strand RNA, but not with the complementary sequences at the 5' terminus of the positive-strand RNA. This interaction was detected by both mobility gel shift and UV cross-linking assays. Furthermore, complex formation exhibited dose-dependency and competition assays confirmed specificity. These results are consistent with our previous observation using the poliovirus 2C protein. The implication of the picornavirus 2C protein binding to the 3'-terminal sequence of the negative-strand untranslated region in viral RNA synthesis is discussed.
引用
收藏
页码:2621 / 2627
页数:7
相关论文
共 36 条
[1]   INDUCTION OF MEMBRANE PROLIFERATION BY POLIOVIRUS PROTEINS 2C AND 2BC [J].
ALDABE, R ;
CARRASCO, L .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 206 (01) :64-76
[2]   A FUNCTIONAL RIBONUCLEOPROTEIN COMPLEX FORMS AROUND THE 5' END OF POLIOVIRUS RNA [J].
ANDINO, R ;
RIECKHOF, GE ;
BALTIMORE, D .
CELL, 1990, 63 (02) :369-380
[3]   POLIOVIRUS RNA-SYNTHESIS UTILIZES AN RNP COMPLEX FORMED AROUND THE 5'-END OF VIRAL-RNA [J].
ANDINO, R ;
RIECKHOF, GE ;
ACHACOSO, PL ;
BALTIMORE, D .
EMBO JOURNAL, 1993, 12 (09) :3587-3598
[4]   Poliovirus-encoded 2C polypeptide specifically binds to the 3'-terminal sequences of viral negative-strand RNA [J].
Banerjee, R ;
Echeverri, A ;
Dasgupta, A .
JOURNAL OF VIROLOGY, 1997, 71 (12) :9570-9578
[5]   Interaction of poliovirus-encoded 2C/2BC polypeptides with the 3′ terminus negative-strand cloverleaf requires an intact stem-loop b [J].
Banerjee, R ;
Tsai, WM ;
Kim, W ;
Dasgupta, A .
VIROLOGY, 2001, 280 (01) :41-51
[6]   Specific interaction of hepatitis C virus protease/helicase NS3 with the 3′-terminal sequences of viral positive- and negative-strand RNA [J].
Banerjee, R ;
Dasgupta, A .
JOURNAL OF VIROLOGY, 2001, 75 (04) :1708-1721
[7]  
BANERJEE R, 2000, RNA VIRUSES PRACTICA, P158
[8]   STRUCTURAL AND FUNCTIONAL-CHARACTERIZATION OF THE POLIOVIRUS REPLICATION COMPLEX [J].
BIENZ, K ;
EGGER, D ;
PFISTER, T ;
TROXLER, M .
JOURNAL OF VIROLOGY, 1992, 66 (05) :2740-2747
[9]   ASSOCIATION OF POLIOVIRAL PROTEINS OF THE P2-GENOMIC REGION WITH THE VIRAL REPLICATION COMPLEX AND VIRUS-INDUCED MEMBRANE SYNTHESIS AS VISUALIZED BY ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY AND AUTORADIOGRAPHY [J].
BIENZ, K ;
EGGER, D ;
PASAMONTES, L .
VIROLOGY, 1987, 160 (01) :220-226
[10]   STRUCTURAL ORGANIZATION OF POLIOVIRUS RNA REPLICATION IS MEDIATED BY VIRAL-PROTEINS OF THE P2 GENOMIC REGION [J].
BIENZ, K ;
EGGER, D ;
TROXLER, M ;
PASAMONTES, L .
JOURNAL OF VIROLOGY, 1990, 64 (03) :1156-1163