CD24 tracks divergent pluripotent states in mouse and human cells

被引：68

作者：

Shakiba, Nika ^{[1
]}

White, Carl A. ^{[1
,2
]}

Lipsitz, Yonatan Y. ^{[1
]}

Yachie-Kinoshita, Ayako ^{[1
,2
]}

Tonge, Peter D. ^{[3
]}

Hussein, Samer M. I. ^{[3
]}

Puri, Mira C. ^{[3
,4
]}

Elbaz, Judith ^{[3
]}

Morrissey-Scoot, James ^{[1
]}

Li, Mira ^{[3
]}

Munoz, Javier ^{[5
,6
,7
]}

Benevento, Marco ^{[5
,6
,7
]}

Rogers, Ian M. ^{[3
,8
,9
]}

Hanna, Jacob H. ^{[10
]}

Heck, Albert J. R. ^{[5
,6
,7
]}

Wollscheid, Bernd ^{[11
,12
,13
]}

Nagy, Andras ^{[3
,9
,14
]}

Zandstra, Peter W. ^{[1
,2
]}

机构：

[1] Univ Toronto, Inst Biomat & Biomed Engn IBBME, Toronto, ON M5S 3E1, Canada

[2] Univ Toronto, Donnelly Ctr Cellular & Biomol Res CCBR, Toronto, ON M5S 3E1, Canada

[3] Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, Toronto, ON M5G 1X5, Canada

[4] Univ Toronto, Dept Med Biophys, Toronto, ON M5T 3H7, Canada

[5] Univ Utrecht, Biomol Mass Spectrometry & Prote, Bijvoet Ctr Biomol Res, 5, NL-3584 CH Utrecht, Netherlands

[6] Univ Utrecht, Utrecht Univ Pharmaceut Sci, 5, NL-3584 CH Utrecht, Netherlands

[7] Netherlands Prote Ctr, NL-3584 CH Utrecht, Netherlands

[8] Univ Toronto, Dept Physiol, Toronto, ON M5S 1A8, Canada

[9] Univ Toronto, Dept Obstet & Gynecol, Toronto, ON M5G 1E2, Canada

[10] Weizmann Inst Sci, Dept Mol Genet, IL-76100 Rehovot, Israel

[11] ETH, Swiss Fed Inst Technol, Inst Mol Syst Biol, Dept Biol, CH-8093 Zurich, Switzerland

[12] ETH, Univ & Swiss Fed Inst Technol, NCCR Neuro Ctr Prote, CH-8093 Zurich, Switzerland

[13] ETH, Swiss Fed Inst Technol, Dept Hlth Sci & Technol, CH-8093 Zurich, Switzerland

[14] Univ Toronto, Inst Med Sci, Toronto, ON M5T 3H7, Canada

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

STEM-CELLS; SOMATIC-CELLS; MOLECULE; DISTINCT; ROUTES; DIFFERENTIATION; QUANTIFICATION; IDENTIFICATION; PROLIFERATION; FIBROBLASTS;

D O I：

10.1038/ncomms8329

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

引用

页数：11

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