Targeting of Mcl-1 Expression by MiRNA-3614-5p Promotes Cell Apoptosis of Human Prostate Cancer Cells

被引：7

作者：

Hsieh, Yi-Hsien ^{[1
,2
]}

Yu, Fang-Jung ^{[3
,4
]}

Nassef, Yasser ^{[1
]}

Liu, Chung-Jung ^{[3
,5
]}

Chen, Yong-Syuan ^{[1
]}

Lin, Ching-Yi ^{[6
]}

Feng, Jia-Liang ^{[7
,8
]}

Wu, Min-Hua ^{[7
,8
]}

机构：

[1] Chung Shan Med Univ, Inst Med, Taichung 40201, Taiwan

[2] Chung Shan Med Univ Hosp, Dept Med Res, Taichung 40201, Taiwan

[3] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Internal Med, Div Gastroenterol, Kaohsiung 80708, Taiwan

[4] Kaohsiung Med Univ, Coll Med, Fac Med, Dept Med, Kaohsiung 80708, Taiwan

[5] Kaohsiung Med Univ, Regenetat Med & Cell Therapy Res Ctr, Kaohsiung 80708, Taiwan

[6] Taichung Vet Gen Hosp, Dept Internal Med, Div Chest Med, Taichung 40705, Taiwan

[7] Cheng Ching Gen Hosp, Lab Dept, Chung Kang Branch, Taichung 40764, Taiwan

[8] Da Yeh Univ, Dept Med Botan & Hlth Applicat, Chunghua 515006, Taiwan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 08期

关键词：

prostate cancer; growth; apoptosis; miRNA-3614-5p; Mcl-1; METASTASIS; MICRORNAS; GROWTH; GENE; LEUKEMIA-1; SUPPRESSES; KNOCKDOWN; MECHANISM; BONE;

D O I：

10.3390/ijms23084194

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNA (miRNA) acts as a critical regulator of growth in various human malignancies. However, the role of miRNA-3614 in the progression of human prostate cancer remains unknown. In this study, our results demonstrated that miRNA-3614-5p exerts a significant inhibitory effect on cell viability and colony formation and induces sub-G1 cell cycle arrest and apoptosis in human prostate cancer cells. Myeloid cell leukemia-1 (Mcl-1) acts as a master regulator of cell survival. Using the miRNA databases, miRNA-3614-5p was found to regulate Mcl-1 expression by targeting positions of the Mcl-1-3 ' UTR. The reduction of Mcl-1 expression by miRNA-3614-5p was further confirmed using an immunoblotting assay. Pro-apoptotic caspase-3 and poly (ADP-ribose) polymerase (PARP) were significantly activated by miRNA-3614-5p to generate cleaved caspase-3 (active caspase-3) and cleaved PARP (active PARP), accompanied by the inhibited Mcl-1 expression. These findings were the first to demonstrate the anti-growth effects of miRNA-3614-5p through downregulating Mcl-1 expression in human prostate cancer cells.

引用

页数：13

共 42 条

[1] MicroRNAs as Guardians of the Prostate: Those Who Stand before Cancer. What Do We Really Know about the Role of microRNAs in Prostate Biology? [J].

Andl, Thomas ;

Ganapathy, Kavya ;

Bossan, Alexia ;

Chakrabarti, Ratna .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (13) :1-33

[2] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[3] The landscape of somatic copy-number alteration across human cancers [J].

Beroukhim, Rameen ;

Mermel, Craig H. ;

Porter, Dale ;

Wei, Guo ;

Raychaudhuri, Soumya ;

Donovan, Jerry ;

Barretina, Jordi ;

Boehm, Jesse S. ;

Dobson, Jennifer ;

Urashima, Mitsuyoshi ;

Mc Henry, Kevin T. ;

Pinchback, Reid M. ;

Ligon, Azra H. ;

Cho, Yoon-Jae ;

Haery, Leila ;

Greulich, Heidi ;

Reich, Michael ;

Winckler, Wendy ;

Lawrence, Michael S. ;

Weir, Barbara A. ;

Tanaka, Kumiko E. ;

Chiang, Derek Y. ;

Bass, Adam J. ;

Loo, Alice ;

Hoffman, Carter ;

Prensner, John ;

Liefeld, Ted ;

Gao, Qing ;

Yecies, Derek ;

Signoretti, Sabina ;

Maher, Elizabeth ;

Kaye, Frederic J. ;

Sasaki, Hidefumi ;

Tepper, Joel E. ;

Fletcher, Jonathan A. ;

Tabernero, Josep ;

Baselga, Jose ;

Tsao, Ming-Sound ;

Demichelis, Francesca ;

Rubin, Mark A. ;

Janne, Pasi A. ;

Daly, Mark J. ;

Nucera, Carmelo ;

Levine, Ross L. ;

Ebert, Benjamin L. ;

Gabriel, Stacey ;

Rustgi, Anil K. ;

Antonescu, Cristina R. ;

Ladanyi, Marc ;

Letai, Anthony .

NATURE, 2010, 463 (7283) :899-905

[4] The tumor suppressor miR-642a-5p targets Wilms Tumor 1 gene and cell-cycle progression in prostate cancer [J].

Beveridge, Dianne J. ;

Richardson, Kirsty L. ;

Epis, Michael R. ;

Brown, Rikki A. M. ;

Stuart, Lisa M. ;

Woo, Andrew J. ;

Leedman, Peter J. .

SCIENTIFIC REPORTS, 2021, 11 (01)

[5] Micro-RNA in pancreatic adenocarcinoma: predictive/prognostic biomarkers or therapeutic targets? [J].

Brunetti, Oronzo ;

Russo, Antonio ;

Scarpa, Aldo ;

Santini, Daniele ;

Reni, Michele ;

Bittoni, Alessandro ;

Azzariti, Amalia ;

Aprile, Giuseppe ;

Delcuratolo, Sabina ;

Signorile, Michele ;

Gnoni, Antonio ;

Palermo, Loredana ;

Lorusso, Vito ;

Cascinu, Stefano ;

Silvestris, Nicola .

ONCOTARGET, 2015, 6 (27) :23323-23341

[6] MicroRNA-195-5p is associated with cell proliferation, migration and invasion in prostate cancer and targets MIB1 [J].

Chen, Bin ;

Bai, Guohui ;

Ma, Xiaoyan ;

Tan, Lulin ;

Xu, Houqiang .

ONCOLOGY REPORTS, 2021, 46 (06)

[7] Modulating the ERK1/2-MMP1 Axis through Corosolic Acid Inhibits Metastasis of Human Oral Squamous Cell Carcinoma Cells [J].

Chen, Jen-Liang ;

Lai, Chung-Yu ;

Ying, Tsung-Ho ;

Lin, Chiao-Wen ;

Wang, Pei-Han ;

Yu, Fang-Jung ;

Liu, Chung-Jung ;

Hsieh, Yi-Hsien .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (16)

[8] Cationic Polymer Nanoparticles-Mediated Delivery of miR-124 Impairs Tumorigenicity of Prostate Cancer Cells [J].

Conte, Raffaele ;

Valentino, Anna ;

Di Cristo, Francesca ;

Peluso, Gianfranco ;

Cerruti, Pierfrancesco ;

Di Salle, Anna ;

Calarco, Anna .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (03)

[9] Study on the mechanism of LOXL1-AS1/miR-3614-5p/YY1 signal axis in the malignant phenotype regulation of hepatocellular carcinoma [J].

Feng, ZhenYu ;

Ye, ZhenYu ;

Xie, JiaMing ;

Chen, Wei ;

Li, Wei ;

Xing, ChunGen .

BIOLOGY DIRECT, 2021, 16 (01)

[10] Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? [J].

Filipowicz, Witold ;

Bhattacharyya, Suvendra N. ;

Sonenberg, Nahum .

NATURE REVIEWS GENETICS, 2008, 9 (02) :102-114

← 1 2 3 4 5 →