Study of DNA Light Switch Ru(II) Complexes: Synthesis, Characterization, Photocleavage and Antimicrobial Activity

被引：20

作者：

Yata, Praveen Kumar ^{[1
]}

Shilpa, M. ^{[1
]}

Nagababu, P. ^{[2
]}

Reddy, M. Rajender ^{[1
]}

Kotha, Laxma Reddy ^{[3
]}

Gabra, Nazar Md ^{[1
]}

Satyanarayana, S. ^{[1
]}

机构：

[1] Osmania Univ, Dept Chem, Hyderabad 500007, Andhra Pradesh, India

[2] Acad Sinica, Inst Chem, Taipei, Taiwan

[3] Univ Nova Lisboa, ITQB, EAN, P-2780157 Oeiras, Portugal

来源：

JOURNAL OF FLUORESCENCE | 2012年 / 22卷 / 03期

关键词：

polypyridyl carboxylic group ligand; Ru(II) complexes; DNA-binding; Molecular light switch; Photocleavage; POLYPYRIDYL RUTHENIUM(II) COMPLEXES; DOUBLE-HELICAL DNA; COPPER(II) COMPLEXES; BINDING PROPERTIES; LIGAND; CLEAVAGE; INTERCALATORS; RECOGNITION; MODE; ACID;

D O I：

10.1007/s10895-011-1018-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The three Ru(II) complexes of [Ru(phen)(2)dppca](2+) (1) [Ru(bpy)(2)dppca](2+) (2) and [Ru(dmb)(2)dppca](2+) (3) (where phen = 1,10 phenanthroline, bpy = 2,2-bipyridine, dmb = 2 ,2-dimethyl 2',2'-bipyridine and polypyridyl ligand containing a single carboxylate functionality dppca ligand (dipyridophenazine-11-carboxylic acid) have been synthesized and characterized. These complexes have been shown to act as promising calf thymus DNA intercalators and a new class of DNA light switches, as evidenced by UV-visible and luminescence titrations with Co2+ and EDTA, steady-state emission quenching by [Fe(CN)(6)](4-) and KI, DNA competitive binding with ethidium bromide, viscosity measurements, and DNA melting experiments. The results suggest that 1, 2, and 3 complexes bind to CT-DNA through intercalation and follows the order 1 > 2 > 3. Under irradiation at 365 nm, the three complexes have also been found to promote the photocleavage of plasmid pBR322 DNA.

引用

页码：835 / 847

页数：13

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