Mesenchymal Stem Cell-Mediated Functional Tooth Regeneration in Swine

被引：891

作者：

Sonoyama, Wataru ^{[3
,4
]}

Liu, Yi ^{[1
,2
]}

Fang, Dianji ^{[1
,2
]}

Yamaza, Takayoshi ^{[3
]}

Seo, Byoung-Moo ^{[5
]}

Zhang, Chunmei ^{[1
,2
]}

Liu, He ^{[6
]}

Gronthos, Stan ^{[7
]}

Wang, Cun-Yu ^{[8
,9
]}

Shi, Songtao ^{[1
,2
]}

Wang, Songlin ^{[3
]}

机构：

[1] Capital Med Univ, Sch Stomatol, Salivary Gland Dis Ctr, Beijing, Peoples R China

[2] Capital Med Univ, Sch Stomatol, Mol Lab Gene Therapy, Beijing, Peoples R China

[3] Univ So Calif, Ctr Craniofacial Mol Biol, Sch Dent, Los Angeles, CA 90033 USA

[4] Okayama Univ, Dept Oral & Maxillofacial Rehabil, Grad Sch Med Dent & Pharmaceut Sci, Okayama, Japan

[5] Seoul Natl Univ, Dept Oral & Maxillofacial Rehabil, Sch Dent, Dent Res Inst, Seoul, South Korea

[6] Peking Univ, Sch Stomatol, Beijing 100871, Peoples R China

[7] Inst Med & Vet Sci, Div Haematol, Mesenchymal Stem Cell Grp, Adelaide, SA 5000, Australia

[8] Univ Michigan, Lab Mol Signaling & Apoptosis, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA

[9] Univ Michigan, Program Mol & Cellular Biol, Ann Arbor, MI 48109 USA

来源：

PLOS ONE | 2006年 / 1卷 / 01期

基金：

中国国家自然科学基金; 美国国家卫生研究院;

关键词：

D O I：

10.1371/journal.pone.0000079

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mesenchymal stem cell-mediated tissue regeneration is a promising approach for regenerative medicine for a wide range of applications. Here we report a new population of stem cells isolated from the root apical papilla of human teeth (SCAP, stem cells from apical papilla). Using a minipig model, we transplanted both human SCAP and periodontal ligament stem cells (PDLSCs) to generate a root/periodontal complex capable of supporting a porcelain crown, resulting in normal tooth function. This work integrates a stem cell-mediated tissue regeneration strategy, engineered materials for structure, and current dental crown technologies. This hybridized tissue engineering approach led to recovery of tooth strength and appearance.

引用

页数：8

共 21 条

[1] High glucose-induced replicative senescence: point of no return and effect of telomerase [J].

Blazer, S ;

Khankin, E ;

Segev, Y ;

Ofir, R ;

Yalon-Hacohen, M ;

Kra-Oz, Z ;

Gottfried, Y ;

Larisch, S ;

Skorecki, KL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 296 (01) :93-101

[2] Prospects for tooth regeneration in the 21st century: A perspective [J].

Chai, Y ;

Slavkin, HC .

MICROSCOPY RESEARCH AND TECHNIQUE, 2003, 60 (05) :469-479

[3] Bioengineered teeth from cultured rat tooth bud cells [J].

Duailibi, MT ;

Duailibi, SE ;

Young, CS ;

Bartlett, JD ;

Vacanti, JP ;

Yelick, PC .

JOURNAL OF DENTAL RESEARCH, 2004, 83 (07) :523-528

[4]

Eckert SE, 2005, INT J ORAL MAX IMPL, V20, P406

[5] Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo [J].

Gronthos, S ;

Mankani, M ;

Brahim, J ;

Robey, PG ;

Shi, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (25) :13625-13630

[6]

Heitz-Mayfield LJA, 2004, INT J ORAL MAX IMPL, V19, P128

[7] Bone formation in vivo: Comparison of osteogenesis by transplanted mouse and human marrow stromal fibroblasts [J].

Krebsbach, PH ;

Kuznetsov, SA ;

Satomura, K ;

Emmons, RVB ;

Rowe, DW ;

Robey, PG .

TRANSPLANTATION, 1997, 63 (08) :1059-1069

[8] Use of the Gottingen minipig as a model of diabetes, with special focus on type 1 diabetes research [J].

Larsen, MO ;

Rolin, B .

ILAR JOURNAL, 2004, 45 (03) :303-313

[9] MELANOMA IN SINCLAIR SWINE - NEW ANIMAL-MODEL [J].

MILLIKAN, LE ;

BOYLON, JL ;

HOOK, RR ;

MANNING, PJ .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1974, 62 (01) :20-30

[10] SHED: Stem cells from human exfoliated deciduous teeth [J].

Miura, M ;

Gronthos, S ;

Zhao, MR ;

Lu, B ;

Fisher, LW ;

Robey, PG ;

Shi, ST .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (10) :5807-5812

← 1 2 3 →