Analyses of effects of α-cembratrien-diol on cell morphology and transcriptome of Valsa mali var. mali

被引：36

作者：

Yan, Ning ^{[1
]}

Du, Yongmei ^{[1
]}

Liu, Xinmin ^{[1
]}

Zhang, Hongbo ^{[1
]}

Liu, Yanhua ^{[1
]}

Shi, John ^{[2
]}

Xue, Sophia Jun ^{[2
]}

Zhang, Zhongfeng ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China

[2] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada

来源：

FOOD CHEMISTRY | 2017年 / 214卷

关键词：

alpha-cembratrien-diol; Valsa mali var. mali; Mycelial morphology; Ultrastructure; RNA-Seq; RNA-SEQ DATA; APPLE; CANKER; QUANTIFICATION; IDENTIFICATION; COLONIZATION; BIOCONTROL; RESPONSES; SEQUENCE; AGENT;

D O I：

10.1016/j.foodchem.2016.07.082

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The objective of this work was to study the underlying mechanisms of growth inhibition of Valsa mali var. mali, the causative pathogen of apple tree canker disease, by alpha-cembratrien-diol. The half maximal inhibitory concentration of alpha-cembratrien-diol against V. mali var. mali is 18.0 mg/L. Treatment of V. mali var. mali with alpha-cembratrien-diol resulted in various mycelial and cellular abnormalities, and the up- and down-regulation of 94 and 170 differentially expressed genes, respectively. Gene Ontology term enrichment analysis revealed that alpha-cembratrien-diol substantially altered the expression of genes involved in the redox process, tetrapyrrole binding, coenzyme binding, heme binding, and iron binding. Kyoto Encyclopedia of Genes and Genomes enrichment analysis also showed significant enrichment of specific metabolic pathways involving the set of differentially expressed genes. The present study will assist in the development of alternative alpha-cembratrien-diol-based biological control agents and ultimately facilitate organic apple production. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：110 / 118

页数：9

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