Competitive protein adsorption to polymer surfaces from human serum

被引:28
作者
Holmberg, Maria [1 ]
Stibius, Karin B. [1 ,3 ]
Larsen, Niels B. [1 ]
Hou, Xiaolin [2 ]
机构
[1] Tech Univ Denmark, Polymer Dept, Riso Natl Lab, DK-4000 Roskilde, Denmark
[2] Tech Univ Denmark, Radiat Res Dept, Riso Natl Lab, DK-4000 Roskilde, Denmark
[3] Univ Copenhagen, Fac Pharmaceut Sci, DK-2100 Copenhagen, Denmark
关键词
D O I
10.1007/s10856-007-3318-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Surface modification by "soft" plasma polymerisation to obtain a hydrophilic and non-fouling polymer surface has been validated using radioactive labelling. Adsorption to unmodified and modified polymer surfaces, from both single protein and human serum solutions, has been investigated. By using different radioisotopes, albumin and Immunoglobulin G (IgG) adsorption has been monitored simultaneously during competitive adsorption processes, which to our knowledge has not been reported in the literature before. Results show that albumin and IgG adsorption is dependent on adsorption time and on the presence and concentration of other proteins in bulk solutions during adsorption. Generally, lower albumin and IgG adsorption was observed on the modified and more hydrophilic polymer surfaces, but otherwise the modified and unmodified polymer surfaces showed the same adsorption characteristics.
引用
收藏
页码:2179 / 2185
页数:7
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