The Philadelphia (Ph; BCR-ABL) chromosome originates from a translocation event between chromosomes 9 and 22, and results in the BCR-ABL fusion gene. In chronic myelogenous leukemia (CML), the BCR-ABL gene is mainly coded for by a major breakpoint cluster region (M-bcr, e13a2 and e14a2). However, in some patients, BCR-ABL genes are encoded by a minor (m)-bcr, e1a2, and a micro (mu)-bcr region, e19a2. These transcripts revealed a different clinical course. The present study described a CML patient whose cytogenetics and FISH analyses of bone marrow revealed a karyotype of 46, XY t(9,22) (q34;q11), while the commercial kits of quantitative PCR (qPCR) failed to detect the BCR-ABL fusion gene. Further multiplex Reverse transcription-PCR (RT-PCR) and sequencing analyses identified a rare e14a3 (b3a3) fusion transcript.
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Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, EnglandUniv London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, England
Barnes, DJ
Melo, JV
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Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, EnglandUniv London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, England
机构:
Johns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Mahidol Univ, Ramathibodi Hosp, Fac Med, Res Ctr, Bangkok 10400, ThailandJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Jinawath, Natini
Norris-Kirby, Alexis
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Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Norris-Kirby, Alexis
Smith, B. Douglas
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Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Smith, B. Douglas
Gocke, Christopher D.
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Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Gocke, Christopher D.
Batista, Denise A.
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Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Batista, Denise A.
Giriffin, Constance A.
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Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA
Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Giriffin, Constance A.
Murphy, Kathleen M.
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Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA
Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
机构:
Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, EnglandUniv London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, England
Barnes, DJ
Melo, JV
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机构:
Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, EnglandUniv London Imperial Coll Sci Technol & Med, Hammersmith Hosp, Royal Postgrad Med Sch, Fac Med,Dep Haematol, London W12 0NN, England
机构:
Johns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Mahidol Univ, Ramathibodi Hosp, Fac Med, Res Ctr, Bangkok 10400, ThailandJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Jinawath, Natini
Norris-Kirby, Alexis
论文数: 0引用数: 0
h-index: 0
机构:
Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Norris-Kirby, Alexis
Smith, B. Douglas
论文数: 0引用数: 0
h-index: 0
机构:
Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Smith, B. Douglas
Gocke, Christopher D.
论文数: 0引用数: 0
h-index: 0
机构:
Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Gocke, Christopher D.
Batista, Denise A.
论文数: 0引用数: 0
h-index: 0
机构:
Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Batista, Denise A.
Giriffin, Constance A.
论文数: 0引用数: 0
h-index: 0
机构:
Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA
Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA
Giriffin, Constance A.
Murphy, Kathleen M.
论文数: 0引用数: 0
h-index: 0
机构:
Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA
Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21205 USAJohns Hopkins Med Inst, Inst Med Genet, Baltimore, MD 21205 USA