DNA adducts of ortho-toluidine in human bladder

被引：17

作者：

Boehm, Francine ^{[1
]}

Schmid, Dagmar ^{[2
]}

Denzinger, Stefan ^{[3
]}

Wieland, Wolf F. ^{[3
]}

Richter, Elmar ^{[1
]}

机构：

[1] Univ Munich, Walther Straub Inst, Dept Toxicol, D-80336 Munich, Germany

[2] Univ Munich, Inst Forens Med, D-80336 Munich, Germany

[3] Univ Regensburg, Dept Urol, Caritas St Josef Med Ctr, Regensburg, Germany

来源：

BIOMARKERS | 2011年 / 16卷 / 02期

关键词：

4-aminobiphenyl; carcinogenicity; gas chromatography; mass spectrometry; smoking; TOBACCO-SPECIFIC NITROSAMINES; CARCINOGENIC AROMATIC-AMINES; METHYLATED ANILINES PRESENT; HEMOGLOBIN ADDUCTS; 4-AMINOBIPHENYL-DNA ADDUCTS; MASS-SPECTROMETRY; CIGARETTE-SMOKE; URINARY-BLADDER; O-ANISIDINE; CANCER;

D O I：

10.3109/1354750X.2010.534556

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Methods: Determination of 4-ABP and o-toluidine-releasing DNA adducts in epithelial and submucosal bladder tissues of sudden death victims (SDV: n == 46), and bladder tumours (n == 12) by gas chromatography/mass spectrometry. Results: Above background, 4 and 11 of 12 tumour samples contained adducts of 4-ABP (0.057 +/-+/- 0.125 fmol/mu A mu g DNA) and o-toluidine (8.72 +/-+/- 4.49 fmol/mu A mu g DNA), respectively. Lower adduct levels were present in both epithelial and submucosal bladder tissues of SDV (4-ABP: 0.011 +/-+/- 0.022 and 0.019 +/-+/- 0.047 fmol/mu A mu g DNA; o-toluidine: 0.24 +/-+/- 0.63 and 0.27 +/-+/- 0.70 fmol/mu A mu g DNA). Conclusion: Detection of o-toluidine-releasing DNA adducts support the carcinogenicity of o-toluidine in the human bladder.

引用

页码：120 / 128

页数：9

共 58 条

[31]

LEWALTER J, 2000, ANAL HAZARDOUS SUBST, V7, P191

[32] ANALYSIS OF 4-AMINOBIPHENYL-DNA ADDUCTS IN HUMAN URINARY-BLADDER AND LUNG BY ALKALINE-HYDROLYSIS AND NEGATIVE-ION GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

LIN, DX ;

LAY, JO ;

BRYANT, MS ;

MALAVEILLE, C ;

FRIESEN, M ;

BARTSCH, H ;

LANG, NP ;

KADLUBAR, FF .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1994, 102 :11-16

[33] Prilocaine plasma levels and methemoglobinemia in patients undergoing tumescent liposuction involving less than 2,000 ml [J].

Lindenblatt, N ;

Belusa, L ;

Tiefenbach, B ;

Schareck, W ;

Olbrisch, RR .

AESTHETIC PLASTIC SURGERY, 2004, 28 (06) :435-440

[34] Synthesis, characterization, and conformational analysis of DNA adducts from methylated anilines present in tobacco smoke [J].

Marques, MM ;

Mourato, LLG ;

Santos, MA ;

Beland, FA .

CHEMICAL RESEARCH IN TOXICOLOGY, 1996, 9 (01) :99-108

[35]

Martone T, 1998, INT J CANCER, V75, P512, DOI 10.1002/(SICI)1097-0215(19980209)75:4<512::AID-IJC4>3.0.CO

[36]

2-W

[37] 32P-postlabeling of N-(deoxyguanosin-8-yl)arylamine adducts:: A comparative study of labeling efficiencies [J].

Mourato, LLG ;

Beland, FA ;

Marques, MM .

CHEMICAL RESEARCH IN TOXICOLOGY, 1999, 12 (07) :661-669

[38] PRIMARY AND SECONDARY-AMINES IN HUMAN ENVIRONMENT [J].

NEURATH, GB ;

DUNGER, M ;

PEIN, FG ;

AMBROSIUS, D ;

SCHREIBER, O .

FOOD AND COSMETICS TOXICOLOGY, 1977, 15 (04) :275-282

[39]

*OECD, 2004, SIDS IN ASS REP O TO

[40] Determination of the levels of aromatic amines in indoor and outdoor air in Italy [J].

Palmiotto, G ;

Pieraccini, G ;

Moneti, G ;

Dolara, P .

CHEMOSPHERE, 2001, 43 (03) :355-361

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