共 28 条
Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex
被引:27
作者:

Choi, Youngseon
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机构:
Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea

Lee, Junghan
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h-index: 0
机构:
Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea

Kim, Keumhyun
论文数: 0 引用数: 0
h-index: 0
机构:
Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea

Kim, Heeyeon
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Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea

Sommer, Peter
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机构:
Inst Pasteur Korea, Cell Biol Retroviruses Lab, Songnam 464400, Gyeonggi Do, South Korea Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea

Song, Rita
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Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea
机构:
[1] Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea
[2] Inst Pasteur Korea, Cell Biol Retroviruses Lab, Songnam 464400, Gyeonggi Do, South Korea
关键词:
RESONANCE ENERGY-TRANSFER;
ENZYMATIC-ACTIVITY;
INHIBITORS;
PROBES;
LINE;
D O I:
10.1039/c0cc02702b
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.
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页码:9146 / 9148
页数:3
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