Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex

被引：27

作者：

Choi, Youngseon ^{[1
]}

Lee, Junghan ^{[1
]}

Kim, Keumhyun ^{[1
]}

Kim, Heeyeon ^{[1
]}

Sommer, Peter ^{[2
]}

Song, Rita ^{[1
]}

机构：

[1] Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea

[2] Inst Pasteur Korea, Cell Biol Retroviruses Lab, Songnam 464400, Gyeonggi Do, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 48期

关键词：

RESONANCE ENERGY-TRANSFER; ENZYMATIC-ACTIVITY; INHIBITORS; PROBES; LINE;

D O I：

10.1039/c0cc02702b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.

引用

页码：9146 / 9148

页数：3

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