Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex

被引:27
作者
Choi, Youngseon [1 ]
Lee, Junghan [1 ]
Kim, Keumhyun [1 ]
Kim, Heeyeon [1 ]
Sommer, Peter [2 ]
Song, Rita [1 ]
机构
[1] Inst Pasteur Korea, Nano Bio Chem Lab, Songnam 464400, Gyeonggi Do, South Korea
[2] Inst Pasteur Korea, Cell Biol Retroviruses Lab, Songnam 464400, Gyeonggi Do, South Korea
关键词
RESONANCE ENERGY-TRANSFER; ENZYMATIC-ACTIVITY; INHIBITORS; PROBES; LINE;
D O I
10.1039/c0cc02702b
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.
引用
收藏
页码:9146 / 9148
页数:3
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