Modified method for determination of hippuric acid and methylhippuric acid in urine by gas chromatography

被引：47

作者：

Kongtip, P

Vararussami, J

Pruktharathikul, V

机构：

[1] Mahidol Univ, Fac Publ Hlth, Dept Occupat Hlth & Safety, Bangkok 10400, Thailand

[2] Dept Pollut Control, Div Air Qual & Noise Management, Bangkok 10400, Thailand

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 751卷 / 01期

关键词：

hippuric acid; methylhippuric acid;

D O I：

10.1016/S0378-4347(00)00463-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modified method for the simultaneous determination of hippuric acid (HA) and o-, m- and p-methylhippuric acids (o-, m- and p-MHAs) in urine is described. These metabolites were extracted, derivatized into their methyl ester derivatives and analyzed using a gas chromatograph equipped with flame ionization detector and a DB-1 capillary column. The derivatives of HA, o-, m- and p-MHAs were well separated within 11 min. The accuracy and precision in the present method were sufficient for quantitative analysis, and the results obtained by the GC method were highly correlated with those by the HPLC method (NIOSH 8301). (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：199 / 203

页数：5

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