Preparation and characterization of 125 I labeled bovine serum albumin

被引：0
|
作者：
Rai, K. S. Ashwitha [1 ]
Jyothi [1 ]
Rasmi, R. R. [1 ]
Sarnaik, Jayula [2 ]
Kadwad, V. B. [2 ]
Shenoy, K. B. [1 ]
Somashekarappa, H. M. [1 ]
机构：
[1] Mangalore Univ, CARRT, Mangalagangothri 574199, Karnataka, India
[2] BRIT, Radiopharmaceut Prod Programme, Vashi 400705, Navi Mumbai, India
来源：
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2015年 / 77卷 / 01期
关键词：
Bovine serum albumin; chloramine-T; radiolabeled; tracer; RADIOIODINATION; PROTEINS;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ( (125) I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 mu g of chloramine-T along with 20 mu g of sodium metabisulphite with 60 seconds incubation at 2 degrees yielded (125) I-BSA with high integrity.
引用
收藏
页码：107 / 110
页数：4
相关论文
共 50 条
  • [41] The novel preparation of 99mTc(I)-labeled human serum albumin (HSA) nanoparticles as a SPECT imaging agent
    Chen, Wan-Jou
    Huang, Feng-Yun J.
    Chang, Hsiu-Yu
    Lee, Te-Wei
    Chang, Chien-Wen
    Lo, Jem-Mau
    JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, 2016, 307 (01) : 141 - 150
  • [42] Preparation and characterization of nanosized P(NIPAM-MBA) hydrogel particles and adsorption of bovine serum albumin on their surface
    Zhu, Xiaoli
    Gu, Xiangling
    Zhang, Lina
    Kong, Xiang-Zheng
    NANOSCALE RESEARCH LETTERS, 2012, 7 : 1 - 8
  • [43] A solid phase adsorption method for preparation of bovine serum albumin-bovine hemoglobin conjugate
    Hu, T
    Su, ZG
    JOURNAL OF BIOTECHNOLOGY, 2003, 100 (03) : 267 - 275
  • [44] Characterization of bovine serum albumin hydrolysates prepared by subcritical water processing
    Koh, Bo-Bae
    Lee, Eun-Jung
    Ramachandraiah, Karna
    Hong, Geun-Pyo
    FOOD CHEMISTRY, 2019, 278 : 203 - 207
  • [45] Characterization of the Interaction Between Esterified TAM Radical and Bovine Serum Albumin
    An Pengjiao
    Yu Nannan
    Sun Ruisheng
    Sui Xiaofang
    Song Yuguang
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2017, 38 (08): : 1354 - 1361
  • [46] Characterization of the interaction between a new merocyanine dye and bovine serum albumin
    Samari, Fayezeh
    Hemmateenejad, Bahram
    Shamsipur, Mojtaba
    Rouhani, Shohre
    Esfandiyari, Fatemeh
    JOURNAL OF THE IRANIAN CHEMICAL SOCIETY, 2016, 13 (12) : 2309 - 2317
  • [47] Characterization of the binding of chrysoidine, an illegal food additive to bovine serum albumin
    Yang, Bingjun
    Hao, Fang
    Li, Jiarong
    Wei, Kai
    Wang, Wenyu
    Liu, Rutao
    FOOD AND CHEMICAL TOXICOLOGY, 2014, 65 : 227 - 232
  • [48] Bovine Serum Albumin Nanoparticles Containing Quercetin: Characterization and Antioxidant Activity
    Antonio, Emilli
    Khalil, Najeh Maissar
    Mainardes, Rubiana Mara
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2016, 16 (02) : 1346 - 1353
  • [49] Characterization of erythrosine B binding to bovine serum albumin and bilirubin displacement
    Mathavan, Vinodaran M. K.
    Boh, Boon Kim
    Tayyab, Saad
    INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 2009, 46 (04) : 325 - 331
  • [50] Characterization of the interaction between a new merocyanine dye and bovine serum albumin
    Fayezeh Samari
    Bahram Hemmateenejad
    Mojtaba Shamsipur
    Shohre Rouhani
    Fatemeh Esfandiyari
    Journal of the Iranian Chemical Society, 2016, 13 : 2309 - 2317
12345