Preparation and characterization of 125 I labeled bovine serum albumin

被引：0
|
作者：
Rai, K. S. Ashwitha [1 ]
Jyothi [1 ]
Rasmi, R. R. [1 ]
Sarnaik, Jayula [2 ]
Kadwad, V. B. [2 ]
Shenoy, K. B. [1 ]
Somashekarappa, H. M. [1 ]
机构：
[1] Mangalore Univ, CARRT, Mangalagangothri 574199, Karnataka, India
[2] BRIT, Radiopharmaceut Prod Programme, Vashi 400705, Navi Mumbai, India
来源：
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2015年 / 77卷 / 01期
关键词：
Bovine serum albumin; chloramine-T; radiolabeled; tracer; RADIOIODINATION; PROTEINS;
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Bovine serum albumin is a model protein, which has been conventionally used as protein standard and in many areas of biochemistry, pharmacology and medicine. Radioiodination procedure for bovine serum albumin employing chloramine-T as an oxidant with slight modification was evaluated critically to establish the optimal conditions for the preparation of radiolabeled tracer ( (125) I-BSA) with required specific activity without impairing the immune reactivity and biological activity. Optimized radioiodination procedure involving 10 mu g of chloramine-T along with 20 mu g of sodium metabisulphite with 60 seconds incubation at 2 degrees yielded (125) I-BSA with high integrity.
引用
收藏
页码：107 / 110
页数：4
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