Genome-wide identification of GAD family genes suggests GhGAD6 functionally respond to Cd2+ stress in cotton

被引：14

作者：

Huang, Hui ^{[1
]}

He, Yunxin ^{[2
]}

Cui, Aihua ^{[3
]}

Sun, Liangqing ^{[1
]}

Han, Mingge ^{[1
]}

Wang, Jing ^{[1
]}

Rui, Cun ^{[1
]}

Lei, Yuqian ^{[1
]}

Liu, Xiaoyu ^{[1
]}

Xu, Nan ^{[1
]}

Zhang, Hong ^{[1
]}

Zhang, Yuexin ^{[1
]}

Fan, Yapeng ^{[1
]}

Feng, Xixian ^{[1
]}

Ni, Kesong ^{[1
]}

Jiang, Jie ^{[2
]}

Zhang, Xingping ^{[2
]}

Chen, Chao ^{[1
]}

Wang, Shuai ^{[1
]}

Chen, Xiugui ^{[1
]}

Lu, Xuke ^{[1
]}

Wang, Delong ^{[1
]}

Wang, Junjuan ^{[1
]}

Yin, Zujun ^{[1
]}

Qaraevna, Bobokhonova Zebinisso ^{[4
]}

Guo, Lixue ^{[1
]}

Zhao, Lanjie ^{[1
]}

Ye, Wuwei ^{[1
]}

机构：

[1] Zhengzhou Univ, Chinese Acad Agr Sci, Sch Agr Sci, State Key Lab Cotton Biol,Inst Cotton Res,Zhengzho, Anyang, Peoples R China

[2] Hunan Inst Cotton Sci, Changde, Peoples R China

[3] Cotton Res Inst Jiangxi Prov, Jiujiang, Peoples R China

[4] Tajik Agr Univ, Dept Cotton Growing Genet Breeding & Seed, Dushanbe, Tajikistan

来源：

FRONTIERS IN GENETICS | 2022年 / 13卷

关键词：

GhGAD6; Cd2+; GABA; expression; evolutionary; GLUTAMATE-DECARBOXYLASE GENES; AMINOBUTYRIC-ACID GABA; CALMODULIN-BINDING; ACCUMULATION; SEQUENCE; OVEREXPRESSION; METABOLISM; EXPRESSION; GROWTH; DOMAIN;

D O I：

10.3389/fgene.2022.965058

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Glutamate decarboxylase (GAD) mainly regulated the biosynthesis of gamma-aminobutyric acid (GABA) and played an important role in plant growth and stress resistance. To explore the potential function of GAD in cotton growth, the genome-wide identification, structure, and expression analysis of GAD genes were performed in this study. There were 10, 9, 5, and 5 GAD genes identified in G. hirsutum, G. barbadense, G. arboreum, and G. raimondii, respectively. GAD was divided into four clades according to the protein motif composition, gene structure, and phylogenetic relationship. The segmental duplication was the main way of the GAD gene family evolution. Most GhGADs respond to abiotic stress. Clade III GAD was induced by Cd2+ stress, especially GhGAD6, and silencing GhGAD6 would lead to more serious Cd2+ poisoning in cotton. The oxidative damage caused by Cd2+ stress was relieved by increasing the GABA content. It was speculated that the decreased expression of GhGAD6 reduced the content of GABA in vivo and caused the accumulation of ROS. This study will further expand our understanding of the relationship between the evolution and function of the GhGAD gene family and provide new genetic resources for cotton breeding under environmental stress and phytoremediation.

引用

页数：17

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