Flow injection-chemiluminescence determination of amoxycillin using potassium permanganate and formaldehyde system

It was found that amoxycillin can react with potassium permanganate in an acidic medium to produce chemiluminescence, which is greatly enhanced by formaldehyde. The optimum conditions for this chemiluminescent reaction were studied in detail using a flow-injection system. The experimental results indicate that, under optimum conditions, the chemiluminescence intensity is linearly related to the concentration of amoxycillin in the range 5.48 x 10(-8)-2.74 x 10(-6) mol/L, with a detection limit (3sigma) of 4.1 x 10(-8) mol/L. The relative standard deviation was 1.0% at 1.1 X 10(-6) mol/L amoxycillin (n = 11 measurements). This method has the advantages of high sensitivity, fast response and ease of operation. The method was successfully applied to the determination of amoxycillin in raw medicines and capsules. Copyright (C) 2005 John Wiley Sons, Ltd.