Simple Sequence Repeat Identification and Endocarp Characterization of Olive Tree Accessions in a Tunisian Germplasm Collection

被引:33
作者
Fendri, Mahdi [1 ]
Trujillo, Isabel [2 ]
Trigui, Ahmed [3 ]
Isabel Rodriguez-Garcia, Maria [1 ]
Alche Ramirez, Juan de Dios [1 ]
机构
[1] CSIC, Dpto Bioquim Biol Celular & Mol Plantas, Estac Expt Zaidin, E-18008 Granada, Spain
[2] Univ Cordoba, Dpto Agron, E-14071 Cordoba, Spain
[3] Inst LOlivier, Sfax 3018, Tunisia
关键词
microsatellite markers; Olea europaea; characterization; varieties; OLEA-EUROPAEA L; GENETIC DIVERSITY; MICROSATELLITE LOCI; CULTIVARS; MARKERS; AFLP; CLASSIFICATION; POLYMORPHISM; VARIABILITY; MANAGEMENT;
D O I
10.21273/HORTSCI.45.10.1429
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Most traditional olive-producing countries possess a diversified genetic patrimony in Olea europaea L. Since the emergence of modern olive growing system, the identification, classification, and conservation of autochthonous olive cultivars is a priority for these countries. In this work, a total of 84 accessions belonging to the "Boughrara"-Sfax olive germplasm collection located in Tunisia have been screened using a powerful set of eight simple sequence repeat markers (SSRs). The study revealed a high genetic variability among the collection and detected a total of 64 alleles. For better management of the mentioned germplasm bank, an improved classification of the entries, including new denominations, has been proposed. In addition, several cases of mislabeling, synonymy, and homonymy have been clarified. Genetic relationships among cultivars have been analyzed showing four major clusters. Finally, a correspondence factor analysis demonstrated that cultivars tend to cluster depending on their main use as oil or table olives. No clear clustering tendencies were observed when the geographical origin of cultivars was used as the criteria for the analysis. All results obtained by SSR screening and classification were in accordance with classification based on morphological traits of fruit endocarps.
引用
收藏
页码:1429 / 1436
页数:8
相关论文
共 49 条
[41]  
SNEATH PHA, 1973, PRINCIPLES PRACTICE, P513
[42]  
Taamalli W., 2008, Journal of Agronomy, V7, P176
[43]   Agronomic and molecular analyses for the characterisation of accessions in Tunisian olive germplasm collections [J].
Taamalli, Wael ;
Geuna, Filippo ;
Banth, Riccardo ;
Bassi, Daniele ;
Daoud, Douja ;
Zarrouk, Mokhtar .
ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2006, 9 (05) :467-481
[44]  
Trigui A., 2006, OLEA, V25, P19
[45]  
TRIGUI A, 2002, OLIVIERS TUNISIE, V1
[46]  
TRUJILLO I, 2005, VARIEDADES OLIVO ESP, P405
[47]  
TRUJILLO T, 1995, J AM SOC HORTIC SCI, V120, P318
[48]   GIMLET:: a computer program for analysing genetic individual identification data [J].
Valière, N .
MOLECULAR ECOLOGY NOTES, 2002, 2 (03) :377-379
[49]   Estimating the probability of identity among genotypes in natural populations: cautions and guidelines [J].
Waits, LP ;
Luikart, G ;
Taberlet, P .
MOLECULAR ECOLOGY, 2001, 10 (01) :249-256