Oxidative degradation of Remazol Turquoise Blue G 133 by soybean peroxidase

被引：50

作者：

Marchis, Tatiana ^{[1
]}

Avetta, Paola ^{[2
]}

Bianco-Prevot, Alessandra ^{[2
]}

Fabbri, Debora ^{[2
]}

Viscardi, Guido ^{[3
]}

Laurenti, Enzo ^{[1
]}

机构：

[1] Univ Torino, Dept Chem, IFM, I-10125 Turin, Italy

[2] Univ Torino, Dept Analyt Chem, I-10125 Turin, Italy

[3] Univ Torino, Dept Gen Chem & Organ Chem, I-10125 Turin, Italy

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2011年 / 105卷 / 02期

关键词：

Reactive dyes; Soybean; Peroxidase; Remazol Blue; WHITE-ROT FUNGI; HORSERADISH-PEROXIDASE; PHTHALOCYANINE DYES; REACTIVE BLACK-5; SYNTHETIC DYES; TEXTILE DYES; DECOLORIZATION; LACCASE; STABILITY; DECOLORATION;

D O I：

10.1016/j.jinorgbio.2010.11.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Reactive dyes are widely employed in textile industries and their removal from wastewaters is a relevant environmental problem. In addition to chemical and physical methods, several bioremediation approaches, involving intact micro-organisms or isolated enzymes, have been proposed to decolorize dye solutions. In this paper, we report the complete and fast decolourization of a Cu(II)-phthalocyanine based reactive dye (Remazol Turquoise Blue G 133) by means of the soybean peroxidase/H2O2 system. The oxidative degradation of the dye in aqueous solution at 25 degrees C was studied as function of pH, revealing a quantitative decolourization yield at acidic pH values with a maximum of activity at pH 3.3. The reaction products were identified and characterized by HPLC-diode array detector (DAD)-mass spectrometry (MS), ionic chromatography and EPR techniques. This analysis showed that the enzyme catalyses the breaking of the phthalocyanine ring producing sulfophthalimide as the main degradation product, and the release of stoichiometric amount of ammonium and Cu(II) ions. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：321 / 327

页数：7

共 38 条

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