Identification of promoters recognized by RNA polymerase-σE holoenzyme from Thermus thermophilus HB8

被引：9
作者：
Shinkai, Akeo
Ohbayashi, Naomi
Terada, Takaho
Shirouzu, Mikako
Kuramitsu, Seiki
Yokoyama, Shigeyuki
机构：
[1] RIKEN, SPring 8 Ctr, Harima Inst, Sayo, Hyogo 6795148, Japan
[2] RIKEN, Yokohama Inst, Genom Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
[3] Osaka Univ, Grad Sch Sci, Dept Biol Sci, Osaka 5600043, Japan
[4] Univ Tokyo, Grad Sch Sci, Dept Biochem & Biophys, Bunkyo Ku, Tokyo 1130033, Japan
来源：
JOURNAL OF BACTERIOLOGY | 2007年 / 189卷 / 23期
关键词：
D O I：
10.1128/JB.01076-07
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Thermus thermophilus sigma(E), an extracytoplasmic function sigma factor from the extremely thermophilic bacterium Thermus thermophilus HB8, bound to the RNA polymerase core enzyme and showed transcriptional activity. With the combination of in vitro transcription assay and GeneChip technology, we identified three promoters recognized by sigma(E). The predicted consensus promoter sequence for sigma(E) is 5'-CA(A/T(A/C)C(A/C)-N-15-CCGTA-3'.
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页码：8758 / 8764
页数：7
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