Combining Single-Molecule Imaging and Single-Channel Electrophysiology

被引：22
|
作者：
Weatherill, Eve E. [1 ]
Wallace, Mark I. [1 ]
机构：
[1] Univ Oxford, Dept Chem, Oxford OX1 3TA, England
来源：
JOURNAL OF MOLECULAR BIOLOGY | 2015年 / 427卷 / 01期
基金：
英国生物技术与生命科学研究理事会; 欧洲研究理事会;
关键词：
single-molecule fluorescence; patch-clamp fluorometry; artificial lipid bilayer; LIPID-BILAYERS; ION CHANNELS; FLUORESCENCE SPECTROSCOPY; RYANODINE RECEPTORS; LIGAND-BINDING; GIANT VESICLES; CA2+ CHANNELS; MEMBRANE; DROPLET; CONDUCTANCE;
D O I：
10.1016/j.jmb.2014.07.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Combining simultaneous single-molecule fluorescence measurements of ion channel conformational change with single-channel electrophysiology would enable a direct link between structure and function. Such methods would help us to create a truly molecular "movie" of how these important biomolecules work. Here we review past and recent progress toward this goal. (C) 2014 Published by Elsevier Ltd.
引用
收藏
页码：146 / 157
页数：12
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