Multi-enzyme logic network architectures for assessing injuries: digital processing of biomarkers

被引：56

作者：

Halamek, Jan ^{[2
]}

Bocharova, Vera ^{[2
]}

Chinnapareddy, Soujanya ^{[2
]}

Windmiller, Joshua Ray ^{[1
]}

Strack, Guinevere ^{[2
]}

Chuang, Min-Chieh ^{[1
]}

Zhou, Jian ^{[2
]}

Santhosh, Padmanabhan ^{[1
]}

Ramirez, Gabriela V. ^{[1
]}

Arugula, Mary A. ^{[2
]}

Wang, Joseph ^{[1
]}

Katz, Evgeny ^{[2
]}

机构：

[1] Univ Calif San Diego, Dept NanoEngn, La Jolla, CA 92093 USA

[2] Clarkson Univ, Dept Chem & Biomol Sci, Potsdam, NY 13699 USA

来源：

MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 12期

关键词：

TRAUMATIC BRAIN-INJURY; MOLECULAR LOGIC; BIOCHEMICAL LOGIC; GATES; ENZYME; INFORMATION; NAND; NOR; NOREPINEPHRINE; FLUORESCENCE;

D O I：

10.1039/c0mb00153h

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.

引用

页码：2554 / 2560

页数：7

共 88 条

[1]

Adamatzky A., 2007, UNCONVENTIONAL COMPU

[2] Smart molecules at work-mimicking advanced logic operations [J].

Andreasson, Joakim ;

Pischel, Uwe .

CHEMICAL SOCIETY REVIEWS, 2010, 39 (01) :174-188

[3] Optimization of Enzymatic Logic Gates and Networks for Noise Reduction and Stability [J].

Arugula, Mary A. ;

Halamek, Jan ;

Katz, Evgeny ;

Melnikov, Dmitriy ;

Pita, Marcos ;

Privman, Vladimir ;

Strack, Guinevere .

2009 SECOND INTERNATIONAL CONFERENCE ON ADVANCES IN CIRCUITS, ELECTRONICS AND MICRO-ELECTRONICS, 2009, :1-+

[4] Boolean logic functions of a synthetic peptide network [J].

Ashkenasy, G ;

Ghadiri, MR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (36) :11140-11141

[5] A molecular switch for biochemical logic gates: Conformational studies [J].

Ashkenazi, G ;

Ripoll, DR ;

Lotan, N ;

Scheraga, HA .

BIOSENSORS & BIOELECTRONICS, 1997, 12 (02) :85-95

[6] Logic gates and elementary computing by enzymes [J].

Baron, Ronan ;

Lioubashevski, Oleg ;

Katz, Eugenii ;

Niazov, Tamara ;

Willner, Itamar .

JOURNAL OF PHYSICAL CHEMISTRY A, 2006, 110 (27) :8548-8553

[7] A molecular NAND gate based on Watson-Crick base pairing [J].

Baytekin, HT ;

Akkaya, EU .

ORGANIC LETTERS, 2000, 2 (12) :1725-1727

[8] An autonomous molecular computer for logical control of gene expression [J].

Benenson, Y ;

Gil, B ;

Ben-Dor, U ;

Adar, R ;

Shapiro, E .

NATURE, 2004, 429 (6990) :423-429

[9] RNA-based computation in live cells [J].

Benenson, Yaakov .

CURRENT OPINION IN BIOTECHNOLOGY, 2009, 20 (04) :471-478

[10] Biocomputers: from test tubes to live cells [J].

Benenson, Yaakov .

MOLECULAR BIOSYSTEMS, 2009, 5 (07) :675-685

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