Proteomic comparison between human young and old brains by two-dimensional gel electrophoresis and identification of proteins

To investigate molecular mechanisms of human brain aging, brain proteins were isolated from postmortem human young and old brains and profiled by two-dimensional gel electrophoresis (2-DE). With the help of special software, five down-regulated protein spots in two-dimensional gel electrophoresis gels of old brains were found compared with young brains, four of which was identified as a protein similar to peroxiredoxin 2 (accession-numbered as gi 11363 1440), two of stathmin (phosphoprotein p19) and apolipoprotein A-I precursor (apo-AI) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight common proteins, whose expressions were not altered between young and old brains, were also identified. The possible relevance of changes was analyzed. This study shows that the contribution of proteomics could be valuable in experimental gerontology field. (C) 2003 ISDN. Published by Elsevier Science Ltd. All rights reserved.