Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination

被引:47
作者
Ashoub, Ahmed [1 ,2 ]
Baeumlisberger, Marion [3 ]
Neupaertl, Moritz [3 ]
Karas, Michael [3 ]
Brueggemann, Wolfgang [1 ,4 ]
机构
[1] Goethe Univ Frankfurt, Inst Ecol Evolut & Divers, D-60438 Frankfurt, Germany
[2] ARC, AGERI, Giza, Egypt
[3] Goethe Univ Frankfurt, Cluster Excellence Macromol Complexes, Inst Pharmaceut Chem, D-60438 Frankfurt, Germany
[4] Biodivers & Climate Res Ctr BiK F, Frankfurt, Germany
关键词
Detoxification; Difference gel electrophoresis; Drought acclimation; Heat shockproteins; Heat stress; Wild barley; 2-DIMENSIONAL ELECTROPHORESIS; MOLECULAR CHAPERONES; RESPONSIVE PROTEINS; GENE-EXPRESSION; SHOCK PROTEINS; COLD STRESS; ARABIDOPSIS; SALINITY; GENOTYPES; TOLERANT;
D O I
10.1007/s11103-015-0291-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In nature, plants are often exposed to combinations of different stresses at the same time, while in many laboratory studies of molecular stress induction phenomena, single stress responses are analyzed. This study aims to identify the common (i.e. more general stress-responsive) and the stress-specific adjustments of the leaf proteome of wild barley to two often co-occurring stress phenomena, i.e. in response to (long-term) drought acclimation (DA) or to (transient) heat stress (HS). In addition, we analyzed those alterations which are specific for the combination of both stresses. Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry with a 1.5 threshold value of changes in relative protein contents. DA resulted in specific upregulation of proteins with cell detoxification functions, water homeostasis maintenance, amino acids synthesis and lipid metabolism and distinct forms of heat shock proteins (HSPs) and proteins with chaperon functions while proteins related to nitrogen metabolism were downregulated. This response was distinguished from the response to transient HS, which included upregulation of a broad range of HSP products. The common response to both stressors revealed upregulation of additional forms of HSPs and the down-regulation of enzymes of the photosynthetic apparatus and chlorophyll binding proteins. The simultaneous exposure to both stress conditions resulted mostly in a combination of both stress responses and to unique abundance changes of proteins with yet unclear functions.
引用
收藏
页码:459 / 471
页数:13
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