Synchronization of pancreatic β-cell rhythmicity after glucagon induction of Ca2+ transients

被引：22

作者：

Grapengiesser, E ^{[1
]}

Dansk, H ^{[1
]}

Hellman, B ^{[1
]}

机构：

[1] Uppsala Univ, Dept Med Cell Biol, Biomedicum, SE-75123 Uppsala, Sweden

来源：

CELL CALCIUM | 2003年 / 34卷 / 01期

关键词：

pancreatic beta-cells; glucagon; fura-2;

D O I：

10.1016/S0143-4160(03)00021-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Pancreatic beta-cells are biological oscillators requiring a coupling force for the synchronization of the cytoplasmic Ca2+ oscillations responsible for pulsatile insulin release. Testing the idea that transients, superimposed on the oscillations, are important for this synchronization, the concentration of cytoplasmic Ca2+ ([Ca2+](i)) was measured with ratiometric fura-2 technique in single beta-cells and small aggregates prepared from islets isolated from ob/ob-mice. Image analyses revealed asynchronous [Ca2+](i) oscillations in adjacent beta-cells lacking physical contact. The addition of glucagon stimulated the firing of [Ca2+](i) transients, which appeared in synchrony in adjacent beta-cells. Moreover, the presence of glucagon promoted synchronization of the [Ca2+](i) oscillations in beta-cells separated by a distance <100 mum but not in those >200 mum apart. The results support the proposal that the repolarizing effect of [Ca2+](i) transients provides a coupling force for co-ordinating the pulses of insulin release generated by pancreatic beta-cells. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：49 / 53

页数：5

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