Challenges in the isolation of taxanes from Taxus canadensis by fast pyrolysis

被引:2
|
作者
Cass, BJ [1 ]
Piskorz, J [1 ]
Scott, DS [1 ]
Legge, RL [1 ]
机构
[1] Univ Waterloo, Dept Chem Engn, Waterloo, ON N2L 3G1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
taxanes; Taxus canadensis; pyrolysis; Taxol; taxaceae;
D O I
10.1016/S0165-2370(00)00148-0
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The Waterloo Fast Pyrolysis Process (WFPP) was used to determine the feasibility of extracting taxanes from needles, twigs and whole clippings of Taxus canadensis. Purification techniques developed for solvent extracts were not suitable for achieving analytic chromatographic separation of taxanes from pyrolytic lignins and other pyrolysis products as these materials are in much higher quantities in pyrolysis oil than in solvent extracts. A number of separation techniques were attempted including liquid-liquid partitioning, silica gel chromatography, adsorption and sodium phenoxide formation. Pretreatment of the feedstock by deionization was also attempted. Taxol(R) was identified in pyrolysis oils of needles and whole clippings with 20% recovery compared to solvent extraction. Other taxanes were most likely present in higher concentrations but could not be analyzed because of interference by phenolic substances. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:275 / 285
页数:11
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