Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study

被引：12

作者：

Kawakubo, Kazumichi ^{[1
]}

Ohnishi, Shunsuke ^{[1
]}

Hatanaka, Yutaka ^{[2
]}

Hatanaka, Kanako C. ^{[2
]}

Hosono, Hidetaka ^{[1
]}

Kubota, Yoshimasa ^{[1
]}

Kamiya, Mako ^{[3
]}

Kuwatani, Masaki ^{[1
]}

Kawakami, Hiroshi ^{[1
]}

Urano, Yasuteru ^{[3
]}

Sakamoto, Naoya ^{[1
]}

机构：

[1] Hokkaido Univ, Dept Gastroenterol & Hepatol, Grad Sch Med, Kita Ku, Kita15 Nishi7, Sapporo, Hokkaido 0608638, Japan

[2] Hokkaido Univ Hosp, Dept Surg Pathol, Sapporo, Hokkaido 060, Japan

[3] Univ Tokyo, Grad Sch Med, Lab Chem Biol & Mol Imaging, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan

来源：

MOLECULAR IMAGING AND BIOLOGY | 2016年 / 18卷 / 03期

关键词：

Endoscopic ultrasound-guided fine needle aspiration; Pancreatic tumor; Fluorescent imaging; Enzymatically activatable fluorescence probe; Rapid on-site evaluation; GAMMA-GLUTAMYL-TRANSPEPTIDASE; ON-SITE EVALUATION; DIAGNOSTIC-ACCURACY; SOLID LESIONS; CANCER-CELLS; FNA; TRANSFERASE; EXPRESSION; BIOPSY; PASSES;

D O I：

10.1007/s11307-015-0898-5

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of gamma-glutamyltranspeptidase (GGT). We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 +/- 7.7 and 7.7 +/- 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05). Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.

引用

页码：463 / 471

页数：9

共 35 条

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