High-performance liquid chromatography with diode array detection method for the simultaneous determination of seven selected phosphodiesterase-5 inhibitors and serotonin reuptake inhibitors used as male sexual enhancers

被引:34
作者
Baker, Mostafa M. [1 ]
Belal, Tarek S. [2 ]
Mahrous, Mohamed S. [3 ]
Ahmed, Hytham M. [4 ]
Daabees, Hoda G. [5 ]
机构
[1] Pharco Pharmaceut Co, Methodol Dept, Alexandria, Egypt
[2] Univ Alexandria, Fac Pharm, Pharmaceut Analyt Chem Dept, Alexandria 21521, Egypt
[3] Univ Alexandria, Fac Pharm, Pharmaceut Chem Dept, Alexandria 21521, Egypt
[4] Damanhour Univ, Fac Pharm, Pharmaceut Anal Dept, Damanhour, Egypt
[5] Damanhour Univ, Fac Pharm, Pharmaceut Chem Dept, Damanhour, Egypt
关键词
Diode array detection; High-performance liquid chromatography; Phosphodiesterase-5; inhibitors; Selective serotonin reuptake inhibitors; Tablet dosage forms; ERECTILE DYSFUNCTION; PREMATURE EJACULATION; ANTIDEPRESSANTS; METABOLITES; VARDENAFIL; TADALAFIL; THERAPY; PLASMA; SILDENAFIL; DAPOXETINE;
D O I
10.1002/jssc.201501339
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This work presents a simple, sensitive and generic high-performance liquid chromatography with diode array detection method for the simultaneous determination of seven drugs prescribed for the treatment of erectile dysfunction and premature ejaculation. Investigated drugs include the phosphodiesterase-5 inhibitors: sildenafil, tadalafil, and vardenafil, in addition to the selective serotonin reuptake inhibitors: dapoxetine, duloxetine, fluoxetine, and paroxetine. The drugs were separated using a Waters C8 column (4.6 x 250 mm, 5 mu m) with the mobile phase consisting of phosphate buffer pH 3, acetonitrile and methanol in the ratio 60:33:7. The flow rate was 1.2 mL/min, and quantification was based on measuring peak areas at 225 nm. Peaks were perfectly resolved with retention times 3.3, 3.9, 6.4, 7.5, 9.5, 10.7, and 13.4 min for vardenafil, sildenafil, paroxetine, duloxetine, dapoxetine, fluoxetine, and tadalafil, respectively. The developed method was validated with respect to system suitability, linearity, ranges, accuracy, precision, robustness, and limits of detection and quantification. The proposed method showed good linearity in the ranges 5-500, 2-200, 2-200, 3-300, 1.5-150, 2-200, and 2-200 mu g/mL for sildenafil, tadalafil, vardenafil, dapoxetine, duloxetine fluoxetine, and paroxetine, respectively. The limits of detection were 0.18-0.38 mu g/mL for the analyzed compounds. The applicability of the proposed method to real life situations was assessed through the analysis of commercial tablets, and satisfactory results were obtained.
引用
收藏
页码:1656 / 1665
页数:10
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