Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells

被引：83

作者：

Chen, Jun ^{[1
,2
]}

Xu, Na ^{[2
]}

Wang, Chenhui ^{[2
]}

Huang, Pin ^{[2
]}

Huang, Huanwei ^{[2
]}

Jin, Zhen ^{[2
]}

Yu, Zhongsheng ^{[3
]}

Cai, Tao ^{[2
]}

Jiao, Renjie ^{[3
,4
]}

Xi, Rongwen ^{[1
,2
,5
]}

机构：

[1] Peking Union Med Coll, Grad Sch, Beijing, Peoples R China

[2] Natl Inst Biol Sci, Beijing, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, Beijing, Peoples R China

[4] Guangzhou Med Univ, Sino French Hoffmann Inst, Guangzhou, Guangdong, Peoples R China

[5] Tongji Univ, Inst Regenerat Med, Shanghai East Hosp, Sch Life Sci & Technol, Shanghai, Peoples R China

来源：

NATURE CELL BIOLOGY | 2018年 / 20卷 / 02期

基金：

中国国家自然科学基金;

关键词：

DROSOPHILA POSTERIOR MIDGUT; OF-SPLIT COMPLEX; TRANSCRIPTION FACTOR; EXPRESSION; PROSPERO; PROGENITOR; PROTEINS; LINEAGE; ACHAETE; GENES;

D O I：

10.1038/s41556-017-0020-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The process through which multiple types of cell-lineage-restricted progenitor cells are specified from multipotent stem cells is unclear. Here we show that, in intestinal stem cell lineages in adult Drosophila, in which the Delta-Notch-signalling-guided progenitor cell differentiation into enterocytes is the default mode, the specification of enteroendocrine cells (EEs) is initiated by transient Scute activation in a process driven by transcriptional self-stimulation combined with a negative feedback regulation between Scute and Notch targets. Scute activation induces asymmetric intestinal stem cell divisions that generate EE progenitor cells. The mitosis-inducing and fate-inducing activities of Scute guide each EE progenitor cell to divide exactly once prior to its terminal differentiation, yielding a pair of EEs. The transient expression of a fate inducer therefore specifies both type and numbers of committed progenitor cells originating from stem cells, which could represent a general mechanism used for diversifying committed progenitor cells from multipotent stem cells.

引用

页码：152 / +

页数：12

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