Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp

被引:108
作者
Zhang, Kai [1 ,2 ,3 ]
Han, Yong-Tao [1 ,2 ,3 ]
Zhao, Feng-Li [1 ,2 ,3 ]
Hu, Yang [1 ,2 ,3 ]
Gao, Yu-Rong [1 ,2 ,3 ]
Ma, Yan-Fei [1 ,2 ,3 ]
Zheng, Yi [4 ]
Wang, Yue-Jin [1 ,2 ,3 ]
Wen, Ying-Qiang [1 ,2 ,3 ]
机构
[1] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China
[2] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China
[3] Minist Agr, Key Lab Hort Plant Biol & Germplasm Innovat North, Yangling 712100, Shaanxi, Peoples R China
[4] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
来源
BMC PLANT BIOLOGY | 2015年 / 15卷
基金
中国国家自然科学基金;
关键词
Grapevine (Vitis vinifera L.) (Vitis pseudoreticulata); CDPK; Synteny analysis; Phylogenetic tree; Subcellular localization; expression profiles; DEPENDENT PROTEIN-KINASE; ABIOTIC STRESS CONDITIONS; ABSCISIC-ACID; CALCIUM; ARABIDOPSIS; RESPONSES; CALMODULIN; TOMATO; ACTIVATION; RESISTANCE;
D O I
10.1186/s12870-015-0552-z
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Calcium-dependent protein kinases (CDPKs) play vital roles in plant growth and development, biotic and abiotic stress responses, and hormone signaling. Little is known about the CDPK gene family in grapevine. Results: In this study, we performed a genome-wide analysis of the 12X grape genome (Vitis vinifera) and identified nineteen CDPK genes. Comparison of the structures of grape CDPK genes allowed us to examine their functional conservation and differentiation. Segmentally duplicated grape CDPK genes showed high structural conservation and contributed to gene family expansion. Additional comparisons between grape and Arabidopsis thaliana demonstrated that several grape CDPK genes occured in the corresponding syntenic blocks of Arabidopsis, suggesting that these genes arose before the divergence of grapevine and Arabidopsis. Phylogenetic analysis divided the grape CDPK genes into four groups. Furthermore, we examined the expression of the corresponding nineteen homologous CDPK genes in the Chinese wild grape (Vitis pseudoreticulata) under various conditions, including biotic stress, abiotic stress, and hormone treatments. The expression profiles derived from reverse transcription and quantitative PCR suggested that a large number of VpCDPKs responded to various stimuli on the transcriptional level, indicating their versatile roles in the responses to biotic and abiotic stresses. Moreover, we examined the subcellular localization of VpCDPKs by transiently expressing six VpCDPK-GFP fusion proteins in Arabidopsis mesophyll protoplasts; this revealed high variability consistent with potential functional differences. Conclusions: Taken as a whole, our data provide significant insights into the evolution and function of grape CDPKs and a framework for future investigation of grape CDPK genes.
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页数:19
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