Application of high-performance anion-exchange chromatography with pulsed amperometric detection and statistical analysis to study oligosaccharide distributions -: a complementary method to investigate the structure and some properties of alginates

被引：32
作者：
Ballance, S
Holtan, S
Aarstad, OA
Sikorski, P
Skjåk-Bræk, G
Christensen, BE
机构：
[1] Norwegian Univ Sci & Technol, NTNU, Norwegian Biopolymer Lab, NOBIPOL,Dept Biotechnol, N-7491 Trondheim, Norway
[2] Norwegian Univ Sci & Technol, NTNU, Dept Phys, NOBIPOL, N-7491 Trondheim, Norway
来源：
JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1093卷 / 1-2期
关键词：
acid hydrolysis; carbohydrate; chain-length distribution; degree of polymerisation;
D O I：
10.1016/j.chroma.2005.07.051
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Alginates comprised of essentially alternating units of mannuronic (M) acid-guluronic (G) acid (MG-alginate), and G-blocks isolated from a seaweed where subjected to partial acid hydrolysis at pH 3.5 The chain-length distribution of oligosaccharides in the hydrolysate were investigated by statistical analysis after their separation with high-performance anion-exchange chromatography and pulsed amperometric detection (HPAEC-PAD). Simulated depolymerisation of the MG-alginate provided an estimate of the ratio between two acid hydrolysis rate constants (p = 8.3 +/- 1) and the average distribution of the MM linkages in the original sample of polysaccharide chains. In conclusion, we found HPAEC-PAD together with statistical analysis was a useful method to investigate the fine structure and some properties of binary polysaccharides. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：59 / 68
页数：10
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