Introduction and expression of the bacterial glyoxylate cycle genes in transgenic mice

被引：8

作者：

Saini, KS ^{[1
]}

Byrne, CR ^{[1
]}

Leish, Z ^{[1
]}

Pruss, CA ^{[1
]}

Rigby, NW ^{[1
]}

Brownlee, AG ^{[1
]}

Nancarrow, CD ^{[1
]}

Ward, KA ^{[1
]}

机构：

[1] CSIRO,DIV ANIM PROD,PROSPECT,NSW 2149,AUSTRALIA

来源：

TRANSGENIC RESEARCH | 1996年 / 5卷 / 06期

关键词：

glyoxylate cycle; isocitrate lyase; malate synthase; microinjection; transgenic mice; expression;

D O I：

10.1007/BF01980212

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The glyoxylate cycle, catalysed by two unique enzymes: isocitrate lyase (ICL; EC 4.1.3.1) and malate synthase (MS; EC 4.1.3.2), is necessary for the net conversion of acetate into glucose. This metabolic pathway operates in microorganisms, higher plants and nematodes. Two bacterial genes, encoding ICL and MS, were modified in order to introduce them into the mouse germ line. The ovine metallothionein-Ia (MT-Ia) promoter-aceB gene-ovine growth hormone (GH) gene (3' GH sequence) construct was fused to the ovine MT-Ia promoter-aceA gene-ovine GH gene (3' GH sequence). Therefore, in this single DNA sequence, both aceA and aceB are under independent MT-Ia promoter control and can be induced by zinc. Transgenic mice were generated by pronuclear microinjection of the aceB-aceA gene construct. We now report the establishment of four mouse lines carying these two transgenes. Studies on the progeny of these lines indicate that one line (No. 91) is expressing both genes at the mRNA and enzyme levels in the liver and intestine, whereas another line (No. 66) has a much lower expression. Both enzyme activities were detected in the liver and intestine at levels up to 25% of those measured in fully derepressed Escherichia coli cells.

引用

页码：467 / 473

页数：7

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