The influence of nitrogen fertilisation on bacterial activity in the rhizosphere of barley

被引：20

作者：

Söderberg, KH ^{[1
]}

Bååth, E ^{[1
]}

机构：

[1] Lund Univ, Dept Microbial Ecol, SE-22362 Lund, Sweden

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2004年 / 36卷 / 01期

关键词：

rhizosphere; bacterial activity; nitrogen fertilisation; Hordeum vulgare;

D O I：

10.1016/j.soilbio.2003.07.001

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

The effect of nitrogen addition on the activity of rhizosphere bacteria was studied using barley seedlings. Three different nitrogen sources were added to the soil (nitrate, ammonium and ammonium + nitrate) at four different concentrations (0, 100, 300 and 500 mg N kg(-1) soil) and the plants were allowed to grow for 6 weeks. The bacterial activity was estimated by measuring thymidine and leucine incorporation into bacteria extracted using homogenisation-centrifugation. Bulk soil bacterial activity was low compared with that of rhizosphere bacteria. Nitrogen addition did not affect the activity of the bulk soil bacteria, indicating that the activity was not nitrogen limited. The thymidine and leucine incorporation rates of rhizosphere bacteria decreased when ammonium or ammonium + nitrate was applied compared with the non-amended controls. No effect on bacterial activity was found following nitrate addition. There was a significant positive correlation between rhizosphere bacterial activity and rhizosphere pH. Shoot length following ammonium treatment was significant lower than in the non-amended control, while nitrate and ammonium + nitrate addition had no effect. This indicates that the varying effects due to nitrogen sources on rhizosphere bacterial activity were not due to effects on plant growth. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：195 / 198

页数：4

共 29 条

[21] ROOT-INDUCED CHANGES IN THE RHIZOSPHERE - IMPORTANCE FOR THE MINERAL-NUTRITION OF PLANTS [J].

MARSCHNER, H ;

ROMHELD, V ;

HORST, WJ ;

MARTIN, P .

ZEITSCHRIFT FUR PFLANZENERNAHRUNG UND BODENKUNDE, 1986, 149 (04) :441-456

[22] Effect of N concentration and N source on root colonization by Pseudomonas fluorescens 2-79RLI [J].

Marschner, P ;

Gerendás, J ;

Sattelmacher, B .

PLANT AND SOIL, 1999, 215 (02) :135-141

[23] Interactions of arbuscular-mycorrhizal fungi and Bacillus strains and their effects on plant growth, microbial rhizosphere activity (thymidine and leucine incorporation) and fungal biomass (ergosterol and chitin) [J].

Medina, A ;

Probanza, A ;

Mañero, FJG ;

Azcón, R .

APPLIED SOIL ECOLOGY, 2003, 22 (01) :15-28

[24] CONVERSION FACTORS FOR ESTIMATION OF CELL PRODUCTION-RATES OF SOIL BACTERIA FROM [H-3] THYMIDINE AND [H-3] LEUCINE INCORPORATION [J].

MICHEL, PH ;

BLOEM, J .

SOIL BIOLOGY & BIOCHEMISTRY, 1993, 25 (07) :943-950

[25]

MORIARTY DJW, 1986, ADV MICROB ECOL, V9, P245

[26] Soil bacteria respond to presence of roots but not to mycelium of arbuscular mycorrhizal fungi [J].

Olsson, PA ;

Baath, E ;

Jakobsen, I ;

Soderstrom, B .

SOIL BIOLOGY & BIOCHEMISTRY, 1996, 28 (4-5) :463-470

[27]

Söderberg KH, 2002, FEMS MICROBIOL ECOL, V40, P223, DOI [10.1016/S0168-6496(02)00233-7, 10.1111/j.1574-6941.2002.tb00955.x]

[28] Bacterial activity along a young barley root measured by the thymidine and leucine incorporation techniques [J].

Soderberg, KH ;

Baath, E .

SOIL BIOLOGY & BIOCHEMISTRY, 1998, 30 (10-11) :1259-1268

[29] Plant species and nutritional-mediated control over rhizodeposition and root decomposition [J].

Van der Krift, TAJ ;

Kuikman, PJ ;

Möller, F ;

Berendse, F .

PLANT AND SOIL, 2001, 228 (02) :191-200

← 1 2 3 →