Optics-intrinsic double-circle phenomenon in protein adsorption visualized by confocal laser scanning microscopy

被引：5

作者：

Yang, Kun ^{[1
]}

Sun, Yan ^{[1
]}

机构：

[1] Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2008年 / 39卷 / 02期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

protein adsorption; confocal laser scanning microscopy; attenuation equation; pore diffusion model;

D O I：

10.1016/j.bej.2007.09.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Confocal laser scanning microscopy (CLSM) has been introduced in the research of chromatographic adsorption and the technique provides a direct and noninvasive way to measure and characterize intraparticle concentration distributions of proteins on a single particle level. In this work, an optics-intrinsic double-circle phenomenon that occurs in protein adsorption visualized by CLSM is exhibited and its optical mechanism is reasonably explained by an evolutive version of the attenuation equation. The time course of intraparticle fluorescence intensity profiles obtained from CLSM (even the double-circle phenomenon) in this research is vividly reproduced by model simulation, which takes into account the light attenuation by combining the attenuation equation with an effective pore diffusion model. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：258 / 266

页数：9

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