Automated multicolor mesoscopic imaging for the 3-dimensional reconstruction of fluorescent biomarker distribution in large tissue specimens

被引:3
作者
Foo, Wanling [1 ]
Wiede, Alexander [2 ,3 ]
Bierwirth, Sebastian [2 ]
Heintzmann, Rainer [2 ,4 ,5 ]
Press, Adrian T. [1 ,3 ,6 ]
Hauswald, Walter [2 ]
机构
[1] Jena Univ Hosp, Dept Anesthesiol & Intens Care Med, Klinikum 1, D-07747 Jena, Germany
[2] Leibniz Res Alliance Leibniz Hlth Technol, Leibniz Inst Photon Technol Leibniz IPHT, Albert Einstein Str 9, D-07745 Jena, Germany
[3] Jena Univ Hosp, Ctr Sepsis Control & Care, Klinikum 1, D-07747 Jena, Germany
[4] Friedrich Schiller Univ, Inst Phys Chem, Helmholtzweg 4, D-07743 Jena, Germany
[5] Friedrich Schiller Univ, Abbe Ctr Photon, Helmholtzweg 4, D-07743 Jena, Germany
[6] Friedrich Schiller Univ, Med Fac, Kastanienstr 1, D-07747 Jena, Germany
关键词
INDOCYANINE GREEN; DRUG; QUANTIFICATION; NANOPARTICLES; MICROSCOPY; DELIVERY; SYSTEM;
D O I
10.1364/BOE.455215
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Research in translational medicine often requires high-resolution characterization techniques to visualize or quantify the fluorescent probes. For example, drug delivery systems contain fluorescent molecules enabling in vitro and in vivo tracing to determine biodistribution or plasma disappearance. Albeit fluorescence imaging systems with sufficient resolution exist, the sample preparation is typically too complex to image a whole organism of the size of a mouse. This article established a mesoscopic imaging technique utilizing a commercially available cryo-microtome and an in-house built episcopic imaging add-on to perform imaging during serial sectioning. Here we demonstrate that our automated red, green, blue (RGB) and fluorescence mesoscope can generate sequential block-face and 3-dimensional anatomical images at variable thickness with high quality of 6 mu m x 6 mu m pixel size. In addition, this mesoscope features a numerical aperture of 0.10 and a field-of-view of up to 21.6 mm x 27 mm x 25 mm (width, height, depth). (C) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
引用
收藏
页码:3723 / 3742
页数:20
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