Novel Evidence that Purinergic Signaling-Nlrp3 Inflammasome Axis Regulates Circadian Rhythm of Hematopoietic Stem/Progenitor Cells Circulation in Peripheral Blood

被引:19
作者
Adamiak, Mateusz [1 ]
Ciechanowicz, Andrzej [1 ]
Skoda, Marta [1 ]
Cymer, Monika [1 ]
Tracz, Michal [2 ]
Xu, Bing [3 ,4 ]
Ratajczak, Mariusz Z. [1 ,5 ]
机构
[1] Med Univ Warsaw, Ctr Preclin Res & Technol, Dept Regenerat Med, Warsaw, Poland
[2] Warsaw Univ Life Sci, Inst Vet Med, Dept Food Hyg & Publ Hlth Protect, Warsaw, Poland
[3] Xiamen Univ, Dept Hematol, Affiliated Hosp 1, Xiamen, Peoples R China
[4] Xiamen Univ, Inst Hematol, Xiamen, Peoples R China
[5] Univ Louisville, Stem Cell Inst, James Graham Brown Canc Ctr, 500 S Floyd St,Rm 107, Louisville, KY 40202 USA
关键词
Diurnal rhythm; Circadian rhythm; Nlrp3; inflammasome; Extracellular ATP; Hematopoietic stem cells; NLRP3; INFLAMMASOME; PROGENITOR CELLS; BONE-MARROW; STEM-CELLS; MOBILIZATION; COMPLEMENT; EXPRESSION; RELEASE; EGRESS; SLEEP;
D O I
10.1007/s12015-020-09953-0
中图分类号
Q813 [细胞工程];
学科分类号
摘要
We found that circadian changes in ATP level in peripheral blood (PB) activate the Nlrp3 inflammasome, which triggers diurnal release of hematopoietic stem/progenitor cells (HSPCs) from murine bone marrow (BM) into PB. Consistent with this finding, we observed circadian changes in expression of mRNA for Nlrp3 inflammasome-related genes, including Nlrp3, caspase 1, IL-1 beta, IL-18, gasdermin (GSDMD), HMGB1, and S100A9. Circadian release of HSPCs from BM into PB as well as expression of Nlrp3-associated genes was decreased in mice in which pannexin 1-mediated secretion of ATP was inhibited by the blocking peptide 10Panx and in animals exposed to the specific small-molecule inhibitor of the Nlrp3 inflammasome MCC950. In addition to HSPCs, a similar decrease in diurnal cell counts was observed for mesenchymal stromal cells (MSCs), endothelial progenitor cells (EPCs), and very small embryonic-like stem cells (VSELs). These results shed more light on the complexity of circadian regulation of HSPC release into PB, which is coordinated in a purinergic signaling-, innate immunity-dependent manner. Moreover, in addition to circadian changes in expression of the Nlrp3 inflammasome we also observed diurnal changes in expression of other inflammasomes, including Aim2, Nrp1a, and Nlrp1b.
引用
收藏
页码:335 / 343
页数:9
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