Effect of Insulin on the Differential Expression of VLDL Receptor Isoforms of SGC7901 Cell and Its Biological Implication

被引：7

作者：

Cai, Zhejun ^{[2
]}

Li, Fei ^{[2
]}

Peng, Chuanmei ^{[2
]}

Li, He ^{[2
]}

Zong, Yiqiang ^{[2
]}

Liu, Zhiguo ^{[1
]}

Qu, Shen ^{[2
]}

机构：

[1] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Biochem & Mol Biol, Wuhan 430074, Peoples R China

来源：

JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES | 2010年 / 30卷 / 05期

关键词：

insulin; very low density lipoprotein receptor; cell proliferation; metabolic regulation; DENSITY-LIPOPROTEIN RECEPTOR; MACROPHAGES; MUSCLE; GROWTH; MICE;

D O I：

10.1007/s11596-010-0541-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study examined the effect of insulin on the expression of very low density lipoprotein receptor (VLDLR) subtypes of SGC7901 cells and discussed its biological implication. In vitro, moderately or poorly-differentiated human gastric adenocarcinoma cell line SGC7901 was incubated with insulin for different lengths of time, and then the expression of protein and RNA level in VLDLR subtypes were detected by Western blotting and real-time PCR, respectively. The results showed that, at certain time interval, insulin could down-regulate expression of type I VLDLR and up-regulate the expression of type II VLDLR in SGC7901 cells, at both protein and RNA level. We are led to conclude that insulin serves as a regulator in maintaining the balance between glucose and lipid metabolism in vivo, possibly through its effect on the differential expression of VLDLR subtypes.

引用

页码：551 / 555

页数：5

共 20 条

[1] Acute suppression of VLDL1 secretion rate by insulin is associated with hepatic fat content and insulin resistance [J].

Adiels, M. ;

Westerbacka, J. ;

Soro-Paavonen, A. ;

Haekkinen, A. M. ;

Vehkavaara, S. ;

Caslake, M. J. ;

Packard, C. ;

Olofsson, S. O. ;

Yki-Jaervinen, H. ;

Taskinen, M. R. ;

Boren, J. .

DIABETOLOGIA, 2007, 50 (11) :2356-2365

[2] Insulin regulation of lipoprotein lipase (LPL) activity and expression in gilthead sea bream (Sparus aurata) [J].

Albalat, A. ;

Saera-Vila, A. ;

Capilla, E. ;

Gutierrez, J. ;

Perez-Sanchez, J. ;

Navarro, I. .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2007, 148 (02) :151-159

[3] Regulation of lipolysis in isolated adipocytes of rainbow trout (Oncorhynchus mykiss):: The role of insulin and glucagon [J].

Albalat, A ;

Gutiérrez, J ;

Navarro, I .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2005, 142 (03) :347-354

[4] PLASMA TRIGLYCERIDE AND CORONARY HEART-DISEASE [J].

AUSTIN, MA .

ARTERIOSCLEROSIS AND THROMBOSIS, 1991, 11 (01) :2-14

[5] CHICKEN OOCYTE GROWTH IS MEDIATED BY AN 8 LIGAND-BINDING REPEAT MEMBER OF THE LDL RECEPTOR FAMILY [J].

BUJO, H ;

HERMANN, M ;

KADERLI, MO ;

JACOBSEN, L ;

SUGAWARA, S ;

NIMPF, J ;

YAMAMOTO, T ;

SCHNEIDER, WJ .

EMBO JOURNAL, 1994, 13 (21) :5165-5175

[6] Role of c-Abl in directing metabolic versus mitogenic effects in insulin receptor signaling [J].

Frasca, Francesco ;

Pandini, Giuseppe ;

Malaguarnera, Roberta ;

Mandarino, Angelo ;

Messina, Rosa Linda ;

Sciacca, Laura ;

Belfiore, Antonino ;

Vigneri, Riccardo .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (36) :26077-26088

[7] Acute hepatic steatosis in mice by blocking β-oxidation does not reduce insulin sensitivity of very-low-density lipoprotein production [J].

Grefhorst, A ;

Hoekstra, J ;

Derks, TGJ ;

Ouwens, DM ;

Baller, JFW ;

Havinga, R ;

Havekes, LM ;

Romijn, JA ;

Kuipers, F .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2005, 289 (03) :G592-G598

[8]

JOKINEN EV, 1994, J BIOL CHEM, V269, P26411

[9]

Kamagate A, 2008, J CLIN INVEST, V118, P2347, DOI [10.1172/JC132914, 10.1172/JCI32914]

[10] Evidence of macrophage foam cell formation by very low-density lipoprotein receptor -: Interferon-γ inhibition of very low-density lipoprotein receptor expression and foam cell formation in macrophages [J].

Kosaka, S ;

Takahashi, S ;

Masamura, K ;

Kanehara, H ;

Sakai, J ;

Tohda, G ;

Okada, E ;

Oida, K ;

Iwasaki, T ;

Hattori, H ;

Kodama, T ;

Yamamoto, T ;

Miyamori, I .

CIRCULATION, 2001, 103 (08) :1142-1147

← 1 2 →