Hypoxia preconditioned bone marrow-derived mesenchymal stromal/stem cells enhance myoblast fusion and skeletal muscle regeneration

被引：51

作者：

Archacka, Karolina ^{[1
]}

Grabowska, Iwona ^{[1
]}

Mierzejewski, Bartosz ^{[1
]}

Graffstein, Joanna ^{[1
]}

Gorzynska, Alicja ^{[1
]}

Krawczyk, Marta ^{[1
]}

Rozycka, Anna M. ^{[1
]}

Kalaszczynska, Ilona ^{[2
,3
]}

Muras, Gabriela ^{[1
]}

Streminska, Wladyslawa ^{[1
]}

Janczyk-Ilach, Katarzyna ^{[1
]}

Walczak, Piotr ^{[4
,5
]}

Janowski, Miroslaw ^{[6
,7
]}

Ciemerych, Maria A. ^{[1
]}

Brzoska, Edyta ^{[1
]}

机构：

[1] Univ Warsaw, Fac Biol, Inst Dev Biol & Biomed Sci, Dept Cytol, Miecznikowa 1 St, PL-02096 Warsaw, Poland

[2] Med Univ Warsaw, Dept Histol & Embryol, PL-02004 Warsaw, Poland

[3] Med Univ Warsaw, Lab Cell Res & Applicat, PL-02097 Warsaw, Poland

[4] Univ Warmia & Mazury, Fac Med Sci, Dept Pathophysiol, Warszawska 30 St, PL-10082 Olsztyn, Poland

[5] Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol & Radiol Sci, Div MR Res, Baltimore, MD 21205 USA

[6] Univ Maryland, Ctr Adv Imaging Res, Dept Diagnost Radiol & Nucl Med, Baltimore, MD 21201 USA

[7] Polish Acad Sci, Mossakowski Med Res Ctr, NeuroRepair Dept, Pawinskiego 5 St, PL-02106 Warsaw, Poland

来源：

STEM CELL RESEARCH & THERAPY | 2021年 / 12卷 / 01期

关键词：

BM-MSC; Fusion; Hypoxia; Normoxia; Migration; Myogenic differentiation; MYOGENIC REGULATORY FACTORS; ADHESION MOLECULE NCAM; SATELLITE CELLS; STEM-CELLS; IN-VITRO; TRANSCRIPTION FACTOR; DIFFERENTIATION; PROLIFERATION; OXYGEN; EXPRESSION;

D O I：

10.1186/s13287-021-02530-3

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Background The skeletal muscle reconstruction occurs thanks to unipotent stem cells, i.e., satellite cells. The satellite cells remain quiescent and localized between myofiber sarcolemma and basal lamina. They are activated in response to muscle injury, proliferate, differentiate into myoblasts, and recreate myofibers. The stem and progenitor cells support skeletal muscle regeneration, which could be disturbed by extensive damage, sarcopenia, cachexia, or genetic diseases like dystrophy. Many lines of evidence showed that the level of oxygen regulates the course of cell proliferation and differentiation. Methods In the present study, we analyzed hypoxia impact on human and pig bone marrow-derived mesenchymal stromal cell (MSC) and mouse myoblast proliferation, differentiation, and fusion. Moreover, the influence of the transplantation of human bone marrow-derived MSCs cultured under hypoxic conditions on skeletal muscle regeneration was studied. Results We showed that bone marrow-derived MSCs increased VEGF expression and improved myogenesis under hypoxic conditions in vitro. Transplantation of hypoxia preconditioned bone marrow-derived MSCs into injured muscles resulted in the improved cell engraftment and formation of new vessels. Conclusions We suggested that SDF-1 and VEGF secreted by hypoxia preconditioned bone marrow-derived MSCs played an essential role in cell engraftment and angiogenesis. Importantly, hypoxia preconditioned bone marrow-derived MSCs more efficiently engrafted injured muscles; however, they did not undergo myogenic differentiation.

引用

页数：18

共 106 条

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