LncRNA XIST promotes liver cancer progression by acting as a molecular sponge of miR-200b-3p to regulate ZEB1/2 expression

被引:25
作者
Liu, Lili [1 ]
Jiang, Hua [2 ]
Pan, Hongming [1 ]
Zhu, Xiuming [1 ,2 ]
机构
[1] Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Med Oncol, Coll Med, Hangzhou, Zhejiang, Peoples R China
[2] Peoples Hosp Hangzhou, Zhejiang Prov Peoples Hosp, Med Coll, Dept Med Oncol, Hangzhou, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Long non-coding RNA; X-inactive specific transcript; miR-200b-3p; zinc finger E-box-binding homeobox 1; 2; liver cancer; metastasis; LONG NONCODING RNA; MESENCHYMAL TRANSITION; EVOLUTION; INVASION; FAMILY; AXIS; EMT;
D O I
10.1177/03000605211016211
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objective To evaluate the predictive value of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST) for survival, and determine the involvement of miRNA(miR)-200b-3p and zinc finger E-box-binding homeobox (ZEB) 1/2 in the pro-tumor effect of lncRNA XIST in liver cancer. Methods We evaluated lncRNA XIST expression in liver cancer tissues and cell lines by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and analyzed the correlation between its expression and overall survival of liver cancer patients by Kaplan-Meier analysis. Its effects on cell proliferation, migration, and invasion were analyzed by Cell-Counting Kit-8 and Transwell assays. The association between lncRNA XIST and miR-200b-3p, and the effects of lncRNA XIST on ZEB1/2 expression were explored using luciferase reporter assays, real-time PCR, and western blotting. Results The lncRNA XIST was significantly upregulated in liver cancer, and increased lncRNA XIST expression was associated with a poor prognosis. The lncRNA XIST promoted liver cancer cell proliferation, migration, and invasion in vitro, and acted as a molecular sponge for miR-200b-3p, and also regulated the expression of ZEB1/2 via miR-200b-3p. Conclusion The lncRNA XIST is an oncogenic lncRNA that promotes liver cancer metastasis, and its pro-metastatic phenotype can be partially attributed to the lncRNA XIST/miR-200b-3p/ZEB1/2 signaling axis.
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页数:12
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