Atomic force microscopy of RecA-DNA complexes using a carbon nanotube tip

被引：37
作者：
Umemura, K
Komatsu, J
Uchihashi, T
Choi, N
Ikawa, S
Nishinaka, T
Shibata, T
Nakayama, Y
Katsura, S
Mizuno, A
Tokumoto, H
Ishikawa, M
Kuroda, R
机构：
[1] Joint Res Ctr Atom Technol, Bio Grp, Tsukuba, Ibaraki 3050046, Japan
[2] Toyohashi Univ Technol, Dept Ecol Engn, Toyohashi, Aichi 4418580, Japan
[3] RIKEN, Inst Phys & Chem Res, Wako, Saitama 3510198, Japan
[4] Univ Osaka Prefecture, Dept Phys & Elect, Sakai, Osaka 5998531, Japan
[5] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 281卷 / 02期
关键词：
RecA; double stranded DNA; carbon nanotube; atomic force microscope;
D O I：
10.1006/bbrc.2001.4333
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report high resolution images of RecA-double stranded (ds) DNA complexes obtained by atomic force microscopy (AFM), When a carbon nanotube (CNT) tip was used, AFM images visualized the 10-nm pitch of RecA-dsDNA complexes and RecA filaments as three-dimensional surface topography without reconstruction analysis. The depth of the notch between two pitches was less than 1 nm, When adsorbed on a soft surface covered with proteins, naked DNA, RecA monomers, RecA hexamers, and short RecA filaments were all clearly resolved in one image. The high resolution images with a CNT tip provided valuable information on the initiation process of RecA-dsDNA complex formation. (C) 2001 Academic Press.
引用
收藏
页码：390 / 395
页数：6
相关论文
共 30 条
[1]   ATOMIC FORCE MICROSCOPE [J].
BINNIG, G ;
QUATE, CF ;
GERBER, C .
PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933
[2]   CIRCULAR DNA-MOLECULES IMAGED IN AIR BY SCANNING FORCE MICROSCOPY [J].
BUSTAMANTE, C ;
VESENKA, J ;
TANG, CL ;
REES, W ;
GUTHOLD, M ;
KELLER, R .
BIOCHEMISTRY, 1992, 31 (01) :22-26
[3]   Atomic force microscopy of single-walled carbon nanotubes using carbon nanotube tip [J].
Choi, N ;
Uchihashi, T ;
Nishijima, H ;
Ishida, T ;
Mizutani, W ;
Akita, S ;
Nakayama, Y ;
Ishikawa, M ;
Tokumoto, H .
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS, 2000, 39 (6B) :3707-3710
[4]   Nanotubes as nanoprobes in scanning probe microscopy [J].
Dai, HJ ;
Hafner, JH ;
Rinzler, AG ;
Colbert, DT ;
Smalley, RE .
NATURE, 1996, 384 (6605) :147-150
[5]   LARGE-SCALE SYNTHESIS OF CARBON NANOTUBES [J].
EBBESEN, TW ;
AJAYAN, PM .
NATURE, 1992, 358 (6383) :220-222
[6]   STRUCTURE OF HELICAL RECA-DNA COMPLEXES .2. LOCAL CONFORMATIONAL-CHANGES VISUALIZED IN BUNDLES OF RECA-ATP-GAMMA-S FILAMENTS [J].
EGELMAN, EH ;
STASIAK, A .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 200 (02) :329-349
[7]   ISOLATION AND VISUALIZATION OF ACTIVE PRESYNAPTIC FILAMENTS OF RECA PROTEIN AND SINGLE-STRANDED-DNA [J].
FLORY, J ;
TSANG, SS ;
MUNIYAPPA, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (22) :7026-7030
[8]   REPRODUCIBLE IMAGING AND DISSECTION OF PLASMID DNA UNDER LIQUID WITH THE ATOMIC FORCE MICROSCOPE [J].
HANSMA, HG ;
VESENKA, J ;
SIEGERIST, C ;
KELDERMAN, G ;
MORRETT, H ;
SINSHEIMER, RL ;
ELINGS, V ;
BUSTAMANTE, C ;
HANSMA, PK .
SCIENCE, 1992, 256 (5060) :1180-1184
[9]   APPLICATIONS FOR ATOMIC-FORCE MICROSCOPY OF DNA [J].
HANSMA, HG ;
LANEY, DE ;
BEZANILLA, M ;
SINSHEIMER, RL ;
HANSMA, PK .
BIOPHYSICAL JOURNAL, 1995, 68 (05) :1672-1677
[10]   IMMOBILIZING DNA ON GOLD VIA THIOL MODIFICATION FOR ATOMIC-FORCE MICROSCOPY IMAGING IN BUFFER SOLUTIONS [J].
HEGNER, M ;
WAGNER, P ;
SEMENZA, G .
FEBS LETTERS, 1993, 336 (03) :452-456
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