Water metabolism in cells of Saccharomyces cerevisiae of races Y-3137 and Y-3327, according to pulsed-field gradient NMR data

被引:10
作者
Avilova, I. A. [1 ]
Vasil'ev, S. G. [1 ]
Rimareva, L. V. [2 ]
Serba, E. M. [2 ]
Volkova, L. D. [1 ,3 ]
Volkov, V. I. [1 ,4 ]
机构
[1] Russian Acad Sci, Inst Problems Chem Phys, Chernogolovka 142432, Moscow Oblast, Russia
[2] Russian Acad Sci, Russian Sci Res Inst Food Biotechnol, Moscow 111033, Russia
[3] Russian State Agr Univ, Timiryazev Moscow Agr Acad, Moscow 127550, Russia
[4] Russian Acad Sci, Ctr Sci, Chernogolovka 142432, Moscow Oblast, Russia
关键词
yeast cells; self-diffusion; pulsed field gradient NMR; permeability; SELF-DIFFUSION; POROUS-MEDIA; COEFFICIENT; BEHAVIOR; PROBE;
D O I
10.1134/S0036024415040020
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The self-diffusion of water in cells of Saccharomyces cerevisiae of races Y-3137 and Y-3327 is studied by means of pulsed-field gradient (PFG) NMR. Three types of water are detected that differ by their self-diffusion coefficients (SDCs): free, intercellular, and intracellular. It is found that the self-diffusion of intercellular and intracellular water is restricted. The size and permeability of the cells of yeasts with different cultivation times (24 and 48 h) is determined by analyzing the dependences of the self-diffusion coefficients of intracellular water on the interval between pulses of the magnetic field gradient.
引用
收藏
页码:710 / 714
页数:5
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