Knowledge on DNA Success Rates to Optimize the DNA Analysis Process: From Crime Scene to Laboratory

被引：55

作者：

Mapes, Anna A. ^{[1
]}

Kloosterman, Ate D. ^{[2
,3
]}

van Marion, Vincent ^{[4
]}

dePoot, Christianne J. ^{[1
,5
]}

机构：

[1] Amsterdam Univ Appl Sci, Forens Sci, Weesperzijde 190, NL-1097 DZ Amsterdam, Netherlands

[2] Netherlands Forens Inst, Dept Sci Interdisciplinary Res Stat & Knowledge M, Laan van Ypenburg 6, NL-2497 GB The Hague, Netherlands

[3] Univ Amsterdam, Forens Biol, NL-1012 WX Amsterdam, Netherlands

[4] Netherlands Forens Inst, Human Biol Traces HBS, Laan van Ypenburg 6, NL-2497 GB The Hague, Netherlands

[5] Dutch Police Acad, Forens Invest, Arnhemseweg 348, NL-7334 AC Apeldoorn, Netherlands

来源：

JOURNAL OF FORENSIC SCIENCES | 2016年 / 61卷 / 04期

关键词：

forensic science; DNA typing; forensic process; scene of crime officer; forensic laboratory; DNA success rates; DEVELOPMENTAL VALIDATION; SYSTEM; DATABASE; SAMPLE; KIT;

D O I：

10.1111/1556-4029.13102

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

DNA analysis has become an essential intelligence tool in the criminal justice system for the identification of possible offenders. However, it appears that about half of the processed DNA samples contains too little DNA for analysis. This study looks at DNA success rates within 28 different categories of trace exhibits and relates the DNA concentration to the characteristics of the DNA profile. Data from 2260 analyzed crime samples show that cigarettes, bloodstains, and headwear have relatively high success rates. Cartridge cases, crowbars, and tie-wraps are on the other end of the spectrum. These objective data can assist forensics in their selection process. The DNA success probability shows a positive relation with the DNA concentration. This finding enables the laboratory to set an evidence-based threshold value in the DNA analysis process. For instance, 958 DNA extracts had a concentration value of 6 pg/mu L or less. Only 46 of the 958 low-level extracts provided meaningful DNA profiling data.

引用

页码：1055 / 1061

页数：7

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