A chronoamperometric method for determining total antioxidant activity

被引：25
作者：
Brainina, Kh. Z. [1 ,2 ]
Varzakova, D. P. [1 ]
Gerasimova, E. L. [1 ,2 ]
机构：
[1] Ural State Univ Econ, Ekaterinburg 620144, Russia
[2] BN Yeltsin Ural Fed Univ, Ekaterinburg 620002, Russia
来源：
JOURNAL OF ANALYTICAL CHEMISTRY | 2012年 / 67卷 / 04期
基金：
俄罗斯基础研究基金会;
关键词：
antioxidant activity; potentiometry; chronoamperometry; mediator system; ASCORBIC-ACID; CAPACITY; ELECTRODE;
D O I：
10.1134/S1061934812020050
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel version of a chronoamperometric method of antioxidant determination is described. It is based on the use of the oxidation current of ferrocyanide formed in the reaction of antioxidants to be determined with potassium ferricyanide as an analytical signal. So the total concentration of all antioxidants present in the analyzed solution, i.e., total antioxidant capacity, is measured. The results of blood plasma analysis by the newly presented and certified potentiometry method are in good agreement with each other, R (2) = 0.9980. The detection limit makes 2 x 10(-6) M for uric and ascorbic acids and 5 x 10(-6) M for cysteine and glutathione.
引用
收藏
页码：364 / 369
页数：6
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